Neuronal Plasma Membrane Dynamics Evoked by Osmomechanical Perturbations
Neuronal Plasma Membrane Dynamics Evoked by Osmomechanical Perturbations
复制标题
渗透力学扰动引起的神经元质膜动力学
DOI：
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发表时间：
1998
影响因子：
2.4
通讯作者：
C. Morris
中科院分区：
文献类型：
--
作者：
L. Mills;C. Morris
抽象的。当神经元膨胀和收缩时，它们广泛地重组它们的质膜。这些膜动力学的一个引人注目的方面是空泡样扩张（VLDs）的短暂出现，这与直觉相反，随着神经元的收缩而扩张。在这里，共聚焦显微镜培养的软体动物神经元与水相和膜染料一起使用，以检查VLD的形式，逆转或恢复的VLD膜拓扑结构的变化。我们发现，VLD开始在粘附表面离散内陷，所以VLD和质膜最初是连续的。在接下来的几分钟内，VLD扩展并穿透细胞质。在基底层，VLD的口部发育成不规则的活动粘附突起环，而在细胞质更深处，VLD膜轮廓是光滑的。随后VLD自发收缩;随着这种恢复的进行，能动的VLD口的收缩导致质膜的内化。用水相染料进行的洗脱实验表明，VLD收缩产生真正的空泡，即，与外部介质隔离的膜结合区室。VLD也可以通过将细胞返回到溶胀条件来实验性地消除;这种逆转过程将膜驱动回到表面。VLD的形成和VLD膜的再内化可以被视为质膜表面积调节的方面。我们假设，区域膜张力差异驱动的区域调整，变得明显时，过度扰动过载正常膜再处理步骤。VLD膜拓扑结构的变化，以及先前建立的电容变化伴随细胞收缩和肿胀，认为渗透力学扰动神经元调节其表面积，因为它们的体积变化。
Abstract. When neurons swell and shrink they extensively reorganize their plasma membrane. A striking aspect of these membrane dynamics is the transient appearance of vacuole-like dilations (VLDs) which, counterintuitively, expand as the neurons shrink. Here, confocal microscopy of cultured molluscan (Lymnaea) neurons was used in conjunction with aqueous phase and membrane dyes to examine changing VLD membrane topology as VLDs form, reverse or recover. We show that VLDs start as discrete invaginations at the adherent surface, so VLD and plasma membranes are initially contiguous. Over the next few minutes VLDs expand and penetrate the cytoplasm. At the substratum, the mouths of VLDs develop into irregular annuli of motile adherent processes whereas deeper in the cytoplasm, VLD membrane profiles are smooth. Subsequently VLDs spontaneously shrink; as this recovery proceeds, constriction of the motile VLD mouth leads to the internalization of plasma membrane. Washout experiments with aqueous phase dyes demonstrated that VLD constriction yields bona fide vacuoles, i.e., membrane-bound compartments isolated from the external medium. VLDs can also be experimentally eliminated by returning cells to swelling conditions; this reversal process drives membrane back to the surface.VLD formation and reinternalization of VLD membrane can be seen as aspects of plasma membrane surface area regulation. We postulate that area adjustments, driven by regional membrane tension differences, become noticeable when excessive perturbations overload normal membrane reprocessing steps. Both the changes in VLD membrane topology, and previously established capacitance changes accompanying cell shrinking and swelling, argue that osmomechanically perturbed neurons regulate their surface area as their volume changes.
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影响因子：
3.3
作者：
Lin,XH;Grako,KA;Burg,MA;Stallcup,WB
通讯作者：
Stallcup,WB
影响因子：
3.4
作者：
S. Heidemann;R. Buxbaum
通讯作者：
S. Heidemann;R. Buxbaum
影响因子：
56.9
作者：
Chen, CS;Mrksich, M;Ingber, DE
通讯作者：
Ingber, DE
影响因子：
4
作者：
Bray,D;Money,NP;Harold,FM;Bamburg,JR
通讯作者：
Bamburg,JR
影响因子：
2.4
作者：
C. Lin;P. Lamoureux;R. Buxbaum;S. Heidemann
通讯作者：
C. Lin;P. Lamoureux;R. Buxbaum;S. Heidemann