Novel Non-Invasive Diagnosis of Bladder Cancer in Urine Based on Multifunctional Nanoparticles.
Novel Non-Invasive Diagnosis of Bladder Cancer in Urine Based on Multifunctional Nanoparticles.
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DOI：
10.3389/fcell.2021.813420
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发表时间：
2021
影响因子：
5.5
通讯作者：
Xu C
中科院分区：
文献类型：
--
作者：
Xu J;Zeng S;Li J;Gao L;Le W;Huang X;Wang G;Chen B;Zhang Z;Xu C
目的：肿瘤细胞由于糖酵解增加而具有永久的表面负电荷，多功能纳米探针(Fe3O4@SiO_2，mNPs)可以通过相反的表面电荷附着在肿瘤细胞上。因此，我们评估了MNPs与尿液混合是否可以提高尿液细胞学检查(UCT)的敏感性。方法：我们开发了一种新的UCT方法，将尿液和mNPs(纳米细胞学)混合在一起，在UCT过程中采集更多的肿瘤细胞。同样的尿液样本被平均分成纳米细胞学和UCT检测，并由细胞病理学家以盲法进行评估。确定了UCT、纳米细胞学以及两者结合(Nano-UCT)检测膀胱癌的准确性。结果：前瞻性采集了长海医院2020年6月至2021年2月102例膀胱癌患者和49例非肿瘤患者的尿样。纳米细胞学检测的总体敏感性显著高于UCT检测(82.4%vs.59.8%，p<；.01)。对于低级别和高级别肿瘤，纳米细胞学和UCT的敏感度分别为79.1%和39.5%(p<；.01)和84.7%和74.6%(p=0.25)。纳米细胞学的特异性略低于UCT(89.8%vs.100%，p=.022)，这主要是由严重的尿路感染引起的。此外，Nano-UCT对所有患者的敏感度为90.2%，对低、高级别肿瘤的敏感度分别为83.7%和94.9%。结论：纳米细胞学检测膀胱癌的敏感性明显高于UCT。它为临床诊断膀胱癌提供了一种很有前途的工具。
Objectives: Tumor cells were reported to have perpetual negative surface charges due to elevated glycolysis, and multifunctional nanoprobes (Fe3O4@SiO2, mNPs) could attach onto tumor cells via opposite surface charges. We thus evaluated whether mixing mNPs with urine could improve the sensitivity of urine cytology test (UCT). Methods: We developed a novel UCT method by mixing urine with mNPs (Nano-cytology) to harvest more tumor cells during UCT procedures. The same voided urine sample was divided equally for the Nano-cytology and UCT assay, and evaluated by cytopathologists in a blinded way. The accuracy of UCT, Nano-cytology, and the combination of the two approaches (Nano-UCT) for detecting bladder cancer were determined. Results: Urine samples were prospectively collected from 102 bladder cancer patients and 49 non-cancer participants from June 2020 to February 2021 in Changhai Hospital. Overall sensitivity of the Nano-cytology assay was significantly higher than that of the UCT assay (82.4 vs. 59.8%, p < .01). Sensitivity for low- and high-grade tumors was 79.1% and 39.5% (p < .01) and 84.7% and 74.6% (p = .25) for Nano-cytology and UCT, respectively. Specificity of Nano-cytology was slightly lower than that of UCT (89.8% vs. 100%, p = .022), which is mainly caused by severe urinary tract infection. In addition, Nano-UCT showed increased sensitivity with 90.2% for overall patients, and 83.7% and 94.9% for low- and high-grade tumor, respectively. Conclusion: The Nano-cytology assay had a significantly improved sensitivity compared with UCT for detecting bladder cancer patients. It represents a promising tool for diagnosis of bladder cancer in clinical practice.
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影响因子：
120.7
作者：
Grossman, HB;Messing, E;Shen, Y
通讯作者：
Shen, Y
影响因子：
2.8
作者：
Eissa, Sanaa;Shawky, Sherif M.;Azzazy, Hassan M. E.
通讯作者：
Azzazy, Hassan M. E.
高转移活性中国膀胱癌细胞系（T921）的建立
DOI：
10.1007/s11626-013-9651-z
发表时间：
2013-10-01
影响因子：
2.1
作者：
Xu, Chuanliang;Xu, Weidong;Sun, Yinghao
通讯作者：
Sun, Yinghao
DOI：
10.2217/nnm.14.212
发表时间：
2015
期刊：
Nanomedicine (London, England)
影响因子：
--
作者：
Tomlinson B;Lin TY;Dall'Era M;Pan CX
通讯作者：
Pan CX
影响因子：
5.6
作者：
Mancini M;Righetto M;Zumerle S;Montopoli M;Zattoni F
通讯作者：
Zattoni F