Shark IgW C region diversification through RNA processing and isotype switching.-猫眼课题宝

DOI：

10.4049/jimmunol.1301257

发表时间：

2013-09-15

期刊：

Journal of immunology (Baltimore, Md. : 1950)

影响因子：

通讯作者：

中科院分区：

其他

文献类型：

作者：

Zhang C;Du Pasquier L;Hsu E

来源链接：

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3839419/?report=classic

Sharks and skates represent the earliest vertebrates with an adaptive immune system based on lymphocyte antigen receptors generated by V(D)J recombination. Shark B cells express two classical immunoglobulins (Ig), IgM and IgW, encoded by an early, alternative gene organization consisting of numerous autonomous miniloci, where the individual gene cluster carries a few rearranging gene segments and one constant region, μ or ω. We have characterized eight distinct Ig miniloci encoding the nurse shark omega heavy (H) chain. Each cluster consists of VH, D and JH segments and six to eight constant (C) domain exons. Two interspersed secretory exons, in addition to the 3’-most C exon with tailpiece, provide the gene cluster with the ability to generate at least six secreted isoforms that differ as to polypeptide length and C domain combination. All clusters appear to be functional, as judged by the capability for rearrangement and absence of defects in the deduced amino acid sequence. We previously showed that IgW VDJ can perform isotype switching to μ C regions; in this study we found that switching also occurs between ω clusters. Thus C region diversification for any IgW VDJ can take place at the DNA level, by switching to other ω or μ C regions, as well as by RNA processing to generate different C isoforms. The wide array of pathogens recognized by antibodies require different disposal pathways, and our findings demonstrate complex and unique pathways for C effector function diversity that evolved independently in cartilaginous fishes.