Compensatory ion transport buffers daily protein rhythms to regulate osmotic balance and cellular physiology.
Compensatory ion transport buffers daily protein rhythms to regulate osmotic balance and cellular physiology.
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DOI：
10.1038/s41467-021-25942-4
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发表时间：
2021-10-15
影响因子：
16.6
通讯作者：
O'Neill JS
中科院分区：
文献类型：
--
作者：
Stangherlin A;Watson JL;Wong DCS;Barbiero S;Zeng A;Seinkmane E;Chew SP;Beale AD;Hayter EA;Guna A;Inglis AJ;Putker M;Bartolami E;Matile S;Lequeux N;Pons T;Day J;van Ooijen G;Voorhees RM;Bechtold DA;Derivery E;Edgar RS;Newham P;O'Neill JS
6%-20%的细胞蛋白质组受到昼夜节律的控制，并根据日常环境周期调节哺乳动物细胞的功能。为了细胞存活，并将体积保持在狭窄的范围内，由于可溶性蛋白质组的变化而造成的渗透势的每日变化必须被抵消。这种渗透补偿的机制和后果以前还没有被研究过。在培养的细胞和组织中，我们发现通过SLC12A家族共转运蛋白的不同活性，代偿涉及Na+，K+和Cl−的电子中和主动运输。在体外和体内的心肌细胞中，代偿性离子通量提供每日变化的电活动。在整个昼夜周期中，可溶蛋白质丰度的扰动对离子组成和细胞功能具有相称的影响。因此，蛋白质组的昼夜节律调节会影响离子的动态平衡，从而对心肌细胞等电活动细胞的生理产生重大影响。电子中和离子转运的渗透补偿缓冲了TORC1介导的胞浆蛋白质组的变化，并在昼夜周期中维持细胞内的动态平衡和细胞体积。在这里，作者发现这种离子含量的变化驱动着心肌细胞电活动的日常节律。
Between 6–20% of the cellular proteome is under circadian control and tunes mammalian cell function with daily environmental cycles. For cell viability, and to maintain volume within narrow limits, the daily variation in osmotic potential exerted by changes in the soluble proteome must be counterbalanced. The mechanisms and consequences of this osmotic compensation have not been investigated before. In cultured cells and in tissue we find that compensation involves electroneutral active transport of Na+, K+, and Cl− through differential activity of SLC12A family cotransporters. In cardiomyocytes ex vivo and in vivo, compensatory ion fluxes confer daily variation in electrical activity. Perturbation of soluble protein abundance has commensurate effects on ion composition and cellular function across the circadian cycle. Thus, circadian regulation of the proteome impacts ion homeostasis with substantial consequences for the physiology of electrically active cells such as cardiomyocytes. Osmotic compensation by electroneutral ion transport buffers TORC1-mediated changes in the cytosolic proteome, and maintains intracellular homeostasis and cell volume over the circadian cycle. Here, the authors find such ion content changes drive daily rhythms in cardiomyocyte electrical activity.
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影响因子：
15
作者：
Derivery E;Bartolami E;Matile S;Gonzalez-Gaitan M
通讯作者：
Gonzalez-Gaitan M
影响因子：
56.9
作者：
Bruening, Franziska;Noya, Sara B.;Robles, Maria S.
通讯作者：
Robles, Maria S.
影响因子：
5.3
作者：
Adragna NC;Ravilla NB;Lauf PK;Begum G;Khanna AR;Sun D;Kahle KT
通讯作者：
Kahle KT
影响因子：
64.5
作者：
Delarue, M.;Brittingham, G. P.;Pfeffer, S.;Surovtsev, I. V.;Pinglay, S.;Kennedy, K. J.;Schaffer, M.;Gutierrez, J. I.;Sang, D.;Poterewicz, G.;Chung, J. K.;Plitzko, J. M.;Groves, J. T.;Jacobs-Wagner, C.;Engel, B. D.;Holt, L. J.
通讯作者：
Holt, L. J.
影响因子：
3.9
作者：
Giovanelli, Emerson;Muro, Eleonora;Lequeux, Nicolas
通讯作者：
Lequeux, Nicolas