Protective effect of blueberry anthocyanins in a CCl4-induced injury model in human embryonic liver cells
Protective effect of blueberry anthocyanins in a CCl4-induced injury model in human embryonic liver cells
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蓝莓花青素对CCl4诱导的人胚胎肝细胞损伤模型的保护作用
DOI：
10.1080/09540105.2013.781139
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发表时间：
2014-02
影响因子：
3
通讯作者：
Zhang, Shi
中科院分区：
文献类型：
--
作者：
Li, Lu-ning;Sun, Ai-dong;Wang, Yin;Zhang, Shi
本研究探讨了蓝莓花色苷对人胚肝L-02细胞的保护作用及其可能的机制。WST-8法结果显示，不同浓度的蓝莓花色苷对CCl 4诱导的人胚肝L-02细胞损伤具有剂量依赖性保护作用，IC 50为6.2×g/L。细胞克隆形成抑制实验表明，随着花色苷浓度的增加，细胞克隆数增加。碘化丙啶（PI）染色分析表明，花青素可使细胞周期阻滞于G1期。DNA倍体分析显示亚二倍体细胞百分比以剂量依赖性方式减弱。此外，Annexin V-FITC/PI染色分析显示花青素对晚期凋亡和坏死细胞具有剂量依赖性作用。Western blot检测结果显示，随着花青素浓度的增加，caspase-3蛋白表达水平逐渐降低。这些结果为蓝莓花色苷的临床应用和对急性肝损伤的保护作用提供了药理学依据。
This study investigates the possible mechanism of the protective effects of blueberry anthocyanins on human embryonic liver L-02 cells. Results of the WST-8 method showed that different concentrations of blueberry anthocyanins protected the human embryonic liver L-02 cells against CCl4-induced injury in a dose-dependent manner with IC50 at 6.2×g/L. Cell clone formation inhibition assay demonstrated that cell clones increased with increased concentration of anthocyanins. Propidium iodide (PI) staining analysis showed that anthocyanins can decrease cell cycle in the G1 phase. DNA ploidy analysis showed a weakened percentage of hypodiploid cells in a dose-dependent manner. Moreover, Annexin V-FITC/PI staining analysis showed a dose-dependent effect of anthocyanins on late apoptotic and necrotic cells. Western blot assay showed gradually decreased caspase-3 protein expression levels with increased anthocyanin concentration. In summary, these findings provided pharmacological evidence supporting the clinical application and protective effect of blueberry anthocyanins against acute liver injury.
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DOI：
10.4049/jimmunol.1101381
发表时间：
2012-01
期刊：
The Journal of Immunology
影响因子：
--
作者：
Li Wang;Kai Zhang;Lianfeng Wu;Shengwu Liu;Honglian Zhang;Qiangjun Zhou;L. Tong;F. Sun;Z. Fan
通讯作者：
Li Wang;Kai Zhang;Lianfeng Wu;Shengwu Liu;Honglian Zhang;Qiangjun Zhou;L. Tong;F. Sun;Z. Fan
影响因子：
6.1
作者：
Matsumoto, H;Nakamura, Y;Hirayama, M
通讯作者：
Hirayama, M
影响因子：
4.5
作者：
P. Tate;Amal Kuzmar;Samuel Smith;D. Wedge;L. Larcom
通讯作者：
P. Tate;Amal Kuzmar;Samuel Smith;D. Wedge;L. Larcom
影响因子：
6.1
作者：
Hsu, Chin-Lin;Huang, Shih-Li;Yen, Gow-Chin
通讯作者：
Yen, Gow-Chin
影响因子：
2
作者：
Bae-jin Ha;Jin Young Lee
通讯作者：
Bae-jin Ha;Jin Young Lee