Exceptionally long CDR3H are not isotype restricted in bovine immunoglobulins.
Exceptionally long CDR3H are not isotype restricted in bovine immunoglobulins.
复制标题
DOI：
10.1371/journal.pone.0064234
复制
发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Diesterbeck US
中科院分区：
文献类型：
--
作者：
Walther S;Czerny CP;Diesterbeck US
异常长的第三互补决定区域的重链（CDR3H）以前被描述为牛IgG和IgM免疫球蛋白的特异性。此外，免疫球蛋白重链位点的基因组组织仍有待阐明，特别关注可变片段（IGHV）的数量。利用dna -PCR技术对变异区进行分析，证实了在所有牛同型中都存在异常长的CDR3H。根据CDR3H的长度对三个不同扩增子的相应序列进行分组。CDR3H序列具有5 ~ 10个、12 ~ 31个或至少48个氨基酸残基。长、中长CDR3H主要由亲水性氨基酸残基组成，短CDR3H还含有疏水性氨基酸残基。所有CDR3H长序列均与种系可变片段10有关。利用牛NCBI构建6.1基因组，分析了牛免疫球蛋白重链位点的基因组结构。研究了BTA21的一个主要位点。编码可变片段、多样性片段和连接片段以及不同同型的恒定区域的外显子也定位在BTA7、BTA8和BTA20上。加上来自未放置的contigs的信息，共检测到36个IGHV，其中13个被认为是功能性的。系统发育分析发现两个牛IGHV家族（boVH1, boVH2）。因此，证明了boVH1包含所有功能片段的两个牛科的存在。这项研究大大提高了对牛免疫球蛋白多样性产生的认识。
Exceptionally long third complementarity determining regions of the heavy chain (CDR3H) were previously described as a specificity of bovine IgG and IgM immunoglobulins. In addition, the genomic organization of the immunoglobulin heavy chain locus remains to be elucidated with a special focus on the number of variable segments (IGHV). By analyzing the variable regions according to the isotype-specific PCR using cDNA-PCR, we were able to prove the existence of exceptional long CDR3H in all bovine isotypes. The corresponding sequences of three distinct amplicons were grouped according to the length of the CDR3H. Sequences of CDR3H possessed 5 to 10, 12 to 31 or at least 48 amino acid residues. Long and mid-length CDR3H were composed of mainly hydrophilic amino acid residues, while short CDR3H also contained hydrophobic amino acid residues. All sequences with long CDR3H were related to the germline variable segment 10. Using the current genome assembly, Bos taurus NCBI build 6.1, the genomic organization of the bovine immunoglobulin heavy-chain locus was analyzed. A main locus was investigated on BTA21. Exons coding for variable, diversity, and joining segments, as well as for the constant regions of different isotypes, were also localized on BTA7, BTA8, and BTA20. Together with the information from unplaced contigs, 36 IGHV were detected of which 13 are putatively functional. Phylogenetic analysis revealed two bovine IGHV families (boVH1, boVH2). Thus, the existence of the two bovine families suggested was demonstrated, where boVH1 comprises all functional segments. This study substantially improves the understanding of the generation of immunoglobulin diversity in cattle.
登录
查看更多内容
影响因子：
15.3
作者：
KUROSAWA, Y;TONEGAWA, S
通讯作者：
TONEGAWA, S
影响因子：
10.5
作者：
HESSE, JE;LIEBER, MR;GELLERT, M
通讯作者：
GELLERT, M
影响因子：
5.8
作者：
Larkin, M. A.;Blackshields, G.;Higgins, D. G.
通讯作者：
Higgins, D. G.
影响因子：
14.9
作者：
Ehrenmann F;Kaas Q;Lefranc MP
通讯作者：
Lefranc MP
影响因子：
64.8
作者：
DESIDERIO, SV;YANCOPOULOS, GD;BALTIMORE, D
通讯作者：
BALTIMORE, D