Three-dimensional visualization of intrauterine conceptus through the uterine wall by tissue clearing method.
Three-dimensional visualization of intrauterine conceptus through the uterine wall by tissue clearing method.
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DOI：
10.1038/s41598-017-06549-6
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发表时间：
2017-07-20
影响因子：
4.6
通讯作者：
Fujiwara H
中科院分区：
文献类型：
--
作者：
Kagami K;Shinmyo Y;Ono M;Kawasaki H;Fujiwara H
三维器官结构中特定细胞的可视化是发展我们对各种器官病理生理机制的认识的关键步骤之一。在这项研究中，我们成功地获得了立体的整体图像的宫内小鼠胚胎和胎盘通过子宫使用改进的组织清除CUBIC方法。通过这个过程，我们可以识别妊娠子宫内各种组织之间的三维关系，并使用计算机系统分析具有单细胞分辨率的横截面的自由角度图像。根据这些数据，我们可以选择最佳的横截面角度，然后产生相应的组织切片，足以进行进一步的免疫组化检查。此外，使用转基因小鼠，可以获得EGFP阳性胚胎和胎盘的清晰图像，确认子宫中胎儿-母体界面中侵入滋养层的精确三维位置。这些结果表明，这一程序将显着有助于分析生殖器官的病理生理机制。
Visualization of specific cells in the three-dimensional organ architecture is one of the key steps to develop our knowledge about pathophysiological mechanisms in various organs. In this study, we successfully obtained stereoscopic whole images of the intrauterine murine embryo and placenta through the uterus using a modified tissue clearing CUBIC method. By this procedure, we can recognize the three-dimensional relationships among various tissues within the pregnant uterus and analyze free-angle images of cross-sections with single-cell resolution using a computer system. Based on these data, we can select optimal cross-section angles and then produce the corresponding tissue slices that are adequate for further immunohistochemical examination. Furthermore, using transgenic mice, distinct images of an EGFP-positive embryo and the placenta can be obtained, confirming the precise three-dimensional location of invading trophoblasts in the feto-maternal interface in the uterus. These results indicate that this procedure will significantly contribute to analyzing pathophysiological mechanisms in reproductive organs.
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