Formation and regeneration of a Wnt-responsive junctional epithelium.
Formation and regeneration of a Wnt-responsive junctional epithelium.
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DOI：
10.1111/jcpe.13371
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发表时间：
2020-12
影响因子：
6.7
通讯作者：
Helms JA
中科院分区：
文献类型：
--
作者：
Yuan X;Chen J;Van Brunt LA;Grauer J;Xu Q;Pei X;Wang L;Zhao Y;Helms JA
目的探讨自我更新结合上皮（JE）持续防御功能的分子机制。采用两种Wnt报告基因小鼠品系Axin 2CreErt 2/+; R26 RmTmG/+和Axin 2LacZ/+，沿着三种临床相关的实验方案，其中JE的功能被破坏：拔牙后、部分牙龈切除术后和完全环周牙龈切除术后。使用转基因Wnt报告基因小鼠品系，我们确定了JE是在其形成时开始的Wnt响应性上皮，并且它将这种状态维持到成年。拔牙后，初始Wnt反应性JE群体的后代直接促进愈合，并最终采用口腔上皮（OE）表型。在传统的部分牙龈切除术模型中，JE完全再生，并且通过原始Wnt响应群体的后代这样做。然而，环牙龈切除术后，OE无法重建功能性JE。在牙齿和口腔上皮细胞之间的界面处的Wnt响应小生境是功能性JE所需的。
To identify the molecular mechanisms mediating the persistent defensive functions of the self-renewing junctional epithelium (JE). Two strains of Wnt reporter mice, Axin2CreErt2/+;R26RmTmG/+ and Axin2LacZ/+, were employed, along with three clinically relevant, experimental scenarios where the function of the JE is disrupted: after tooth extraction, after a partial gingivectomy and after a complete circumferential gingivectomy. Using transgenic Wnt reporter strains of mice, we established the JE is a Wnt-responsive epithelium beginning at the time of its formation, and that it maintains this status into adulthood. After tooth extraction, progeny of the initial Wnt-responsive JE population directly contributed to healing, and ultimately adopted an oral epithelium (OE) phenotype. In the traditional partial gingivectomy model, the JE completely regenerated and did so via progeny of the original Wnt-responsive population. However, following circumferential gingivectomy, the OE was incapable of reestablishing a functional JE. A Wnt-responsive niche at the interface between tooth and oral epithelia is required for a functional JE.
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连接上皮起源于爆发的牙齿的牙源性上皮。
影响因子：
4.6
作者：
Yajima-Himuro S;Oshima M;Yamamoto G;Ogawa M;Furuya M;Tanaka J;Nishii K;Mishima K;Tachikawa T;Tsuji T;Yamamoto M
通讯作者：
Yamamoto M
影响因子：
7.3
作者：
Heyman O;Koren N;Mizraji G;Capucha T;Wald S;Nassar M;Tabib Y;Shapira L;Hovav AH;Wilensky A
通讯作者：
Wilensky A
影响因子：
5.3
作者：
Lustig, B;Jerchow, B;Behrens, J
通讯作者：
Behrens, J
影响因子：
5.8
作者：
Yuan, Xue;Xu, Quanchen;Helms, Jill A.
通讯作者：
Helms, Jill A.
影响因子：
7.6
作者：
Oksanen, J;Sorokin, LM;Hormia, M
通讯作者：
Hormia, M