The Botrytis cinerea Crh1 transglycosylase is a cytoplasmic effector triggering plant cell death and defense response.
The Botrytis cinerea Crh1 transglycosylase is a cytoplasmic effector triggering plant cell death and defense response.
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灰霉病菌Crh1转糖基酶是一种细胞质效应因子，可触发植物细胞死亡和防御反应。
DOI：
10.1038/s41467-021-22436-1
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发表时间：
2021-04-12
影响因子：
16.6
通讯作者：
Sharon A
中科院分区：
文献类型：
--
作者：
Bi K;Scalschi L;Jaiswal N;Mengiste T;Fried R;Sanz AB;Arroyo J;Zhu W;Masrati G;Sharon A
Crh蛋白催化真菌细胞壁中几丁质和葡聚糖聚合物的交联。在这里，我们表明，BcCrh 1蛋白从植物病原真菌灰葡萄孢作为细胞质效应和植物防御的激发子。BcCrh 1定位于空泡和内质网在saperitic生长。然而，在植物感染时，蛋白质在感染垫中积累;然后分泌到质外体并易位到植物细胞中，在那里它诱导细胞死亡和防御反应。53和35个氨基酸的两个区域分别足以用于蛋白质摄取和细胞死亡诱导。不能二聚化的BcCrh 1突变体缺乏转糖基化活性，但仍然能够诱导植物细胞死亡。此外，表达bccrh 1基因的拟南芥品系表现出对B的敏感性降低。灰霉病，这表明了潜在的使用BcCrh 1蛋白在植物免疫对这种坏死型病原体。Crh蛋白催化真菌细胞壁中几丁质和葡聚糖聚合物的交联。在此，Bi等人表明，来自植物病原真菌灰葡萄孢（Botrytis cinerea）的Crh蛋白充当植物防御的细胞质效应子和激发子，并且表达该基因的植物表现出对病原体的敏感性降低。
Crh proteins catalyze crosslinking of chitin and glucan polymers in fungal cell walls. Here, we show that the BcCrh1 protein from the phytopathogenic fungus Botrytis cinerea acts as a cytoplasmic effector and elicitor of plant defense. BcCrh1 is localized in vacuoles and the endoplasmic reticulum during saprophytic growth. However, upon plant infection, the protein accumulates in infection cushions; it is then secreted to the apoplast and translocated into plant cells, where it induces cell death and defense responses. Two regions of 53 and 35 amino acids are sufficient for protein uptake and cell death induction, respectively. BcCrh1 mutant variants that are unable to dimerize lack transglycosylation activity, but are still able to induce plant cell death. Furthermore, Arabidopsis lines expressing the bccrh1 gene exhibit reduced sensitivity to B. cinerea, suggesting a potential use of the BcCrh1 protein in plant immunization against this necrotrophic pathogen. Crh proteins catalyze crosslinking of chitin and glucan polymers in fungal cell walls. Here, Bi et al. show that a Crh protein from the phytopathogenic fungus Botrytis cinerea acts as a cytoplasmic effector and elicitor of plant defense, and plants expressing this gene exhibit reduced sensitivity to the pathogen.
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DOI：
10.1007/978-1-4939-1363-3_1
发表时间：
2014-01-01
期刊：
YEAST GENETICS: METHODS AND PROTOCOLS
影响因子：
--
作者：
Gietz, R. Daniel
通讯作者：
Gietz, R. Daniel
影响因子：
6.7
作者：
Lenarcic, Tea;Pirc, Katja;Anderluh, Gregor
通讯作者：
Anderluh, Gregor
影响因子：
3.5
作者：
Chagué, V;Danit, LV;Sharon, A
通讯作者：
Sharon, A
影响因子：
11.6
作者：
Dou, Daolong;Kale, Shiv D.;Tyler, Brett M.
通讯作者：
Tyler, Brett M.
影响因子：
7.2
作者：
Kars, I;Krooshof, GH;van Kan, JAL
通讯作者：
van Kan, JAL