A metabarcoding comparison of taxonomic richness and composition between the water column and the benthic boundary layer

A metabarcoding comparison of taxonomic richness and composition between the water column and the benthic boundary layer
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水体和底栖边界层之间分类丰富度和组成的元条形码比较

DOI:
10.1093/icesjms/fsaa228
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发表时间:
2021
影响因子:
3.3
通讯作者:
Parry H
Parry H
中科院分区:
农林科学2区
文献类型:
--
作者:
Parry H

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在陆架海进行浮游动物监测主要使用渔网,而渔网无法覆盖海床上方的底栖边界层。然而,这一水层和底栖组合之间的边界可以是丰富的动物区系,有自己独特的超底栖动物群,并作为越冬或昼夜迁徙浮游动物的低光避难所。为了比较浮游生物和BBL栖息地之间的物种丰富度和组成,我们在西英吉利海峡季节性采样的长期监测网站。元条码方法应用于垂直网样品(顶部50米,在一个54米的水柱)和那些从超底栖雪橇产生了超过100 000序列聚类成294个业务分类单位。其中,215个在BBL中,170个在水柱中。一些关键的分类群(如糠虾)是原生于BBL;相比之下,其他微妙的分类群(如栉水母)似乎避免了BBL。浮游生物组成的主要对比与季节性周期有关,而不是与浮游生物BBL差异有关,这表明该网站的基本动态是由我们正在进行的长期每周分辨率监测捕获的。总体而言,元条码的方法,适用于水柱和BBL,提供了一个独立的观点浮游生物动态,并增强现有的传统方法。
Zooplankton monitoring in shelf seas predominantly uses nets that miss the benthic boundary layer (BBL) just above the seabed. However, this boundary between pelagic and benthic assemblages can be faunistically rich, having its own distinct hyperbenthic fauna and acting as a low-light refuge for overwintering or dielly migrating zooplankton. To compare species richness and composition between pelagic and BBL habitats, we sampled a long-term monitoring site in the Western English Channel seasonally. Metabarcoding methods applied to vertical net samples (top 50 m in a ∼54-m water column) and those from a hyperbenthic sledge generated >100 000 sequences clustered into 294 operational taxonomic units. Of these, 215 were found in the BBL and 170 in the water column. Some key taxa (e.g. mysids) were native to the BBL; by contrast, other delicate taxa (e.g. ctenophores) seemed to avoid the BBL. The major contrasts in plankton composition related to the seasonal cycle rather than to pelagic-BBL differences, suggesting that the basic dynamics of the site are captured by our ongoing long-term weekly resolution monitoring. Overall, metabarcoding approaches, applied to both water column and BBL, provide an independent view of plankton dynamics, and augment existing traditional methods.
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DOI: 10.1093/plankt/23.9.903
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影响因子: 2.1
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期刊: Hydrobiologia
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