Relationship between the gut microbiota and bile acid composition in the ileal mucosa of Crohn's disease.
Relationship between the gut microbiota and bile acid composition in the ileal mucosa of Crohn's disease.
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克罗恩病回肠黏膜中肠道微生物群与胆汁酸组成之间的关系
DOI：
10.5217/ir.2021.00054
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发表时间：
2022-07
影响因子：
4.9
通讯作者：
Andoh, Akira
中科院分区：
文献类型：
--
作者：
Bamba, Shigeki;Inatomi, Osamu;Nishida, Atsushi;Ohno, Masashi;Imai, Takayuki;Takahashi, Kenichiro;Naito, Yuji;Iwamoto, Junichi;Honda, Akira;Inohara, Naohiro;Andoh, Akira
肠道菌群与胆汁酸之间的相互作用在胃肠道疾病的发病机制中起着重要作用。我们研究了克罗恩病（CD）回肠粘膜微生物结构与胆汁酸代谢之间的关系。入选了12名非CD对照和38名临床缓解的CD患者。在球囊辅助肠镜下从回肠远端采集样本。采用液相色谱-质谱联用法分析胆汁酸组成。通过16 S rRNA基因测序分析肠道微生物群。香农均匀度指数显着低于内镜下活动性病变比非CD对照。通过UniFrac指标评价的β多样性显示活动性病变与非CD对照之间存在显著差异（P=0.039）。CD样品中埃希氏菌的相对丰度显著高于非CD对照，而粪杆菌属和罗斯拜瑞氏菌属的相对丰度显著低于非CD对照。大肠埃希氏菌丰度的增加在活动性病变中比在非活动性病变中更突出。CD患者的结合胆汁酸比例显著高于非CD对照组，但原发性或继发性胆汁酸比例无差异。大肠杆菌属和乳酸杆菌属与结合胆汁酸的比例呈正相关。另一方面，罗斯拜瑞氏菌属、肠杆菌属和粪杆菌属与结合胆汁酸的比例呈负相关。在CD患者的回肠中发现了粘膜相关的生态失调和胆汁酸组成的改变。这些可能在CD患者回肠病变的病理生理学中发挥作用。
Crosstalk between the gut microbiota and bile acid plays an important role in the pathogenesis of gastrointestinal disorders. We investigated the relationship between microbial structure and bile acid metabolism in the ileal mucosa of Crohn’s disease (CD). Twelve non-CD controls and 38 CD patients in clinical remission were enrolled. Samples were collected from the distal ileum under balloon-assisted enteroscopy. Bile acid composition was analyzed by liquid chromatography-mass spectrometry. The gut microbiota was analyzed by 16S rRNA gene sequencing. The Shannon evenness index was significantly lower in endoscopically active lesions than in non-CD controls. β-Diversity, evaluated by the UniFrac metric, revealed a significant difference between the active lesions and non-CD controls (P=0.039). The relative abundance of Escherichia was significantly higher and that of Faecalibacterium and Roseburia was significantly lower in CD samples than in non-CD controls. The increased abundance of Escherichia was more prominent in active lesions than in inactive lesions. The proportion of conjugated bile acids was significantly higher in CD patients than in non-CD controls, but there was no difference in the proportion of primary or secondary bile acids. The genera Escherichia and Lactobacillus were positively correlated with the proportion of conjugated bile acids. On the other hand, Roseburia, Intestinibacter, and Faecalibacterium were negatively correlated with the proportion of conjugated bile acids. Mucosa-associated dysbiosis and the alteration of bile acid composition were identified in the ileum of CD patients. These may play a role in the pathophysiology of ileal lesions in CD patients.
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DOI：
10.1038/nri.2016.88
发表时间：
2016-10
期刊：
Nature reviews. Immunology
影响因子：
--
作者：
Johansson ME;Hansson GC
通讯作者：
Hansson GC
影响因子：
30.3
作者：
Sinha SR;Haileselassie Y;Nguyen LP;Tropini C;Wang M;Becker LS;Sim D;Jarr K;Spear ET;Singh G;Namkoong H;Bittinger K;Fischbach MA;Sonnenburg JL;Habtezion A
通讯作者：
Habtezion A
影响因子：
4.4
作者：
Schloss, Patrick D.;Westcott, Sarah L.;Weber, Carolyn F.
通讯作者：
Weber, Carolyn F.
影响因子：
4.9
作者：
Frank, Daniel N.;Robertson, Charles E.;Hamm, Christina M.;Kpadeh, Zegbeh;Zhang, Tianyi;Chen, Hongyan;Zhu, Wei;Sartor, R. Balfour;Boedeker, Edgar C.;Harpaz, Noam;Pace, Norman R.;Li, Ellen
通讯作者：
Li, Ellen
影响因子：
64.8
作者：
Lloyd-Price, Jason;Arze, Cesar;Yajnik, Vijay
通讯作者：
Yajnik, Vijay