Human Meibum and Tear Film Derived (O-Acyl)-Omega-Hydroxy Fatty Acids as Biomarkers of Tear Film Dynamics in Meibomian Gland Dysfunction and Dry Eye Disease.
Human Meibum and Tear Film Derived (O-Acyl)-Omega-Hydroxy Fatty Acids as Biomarkers of Tear Film Dynamics in Meibomian Gland Dysfunction and Dry Eye Disease.
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DOI：
10.1167/iovs.62.9.13
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发表时间：
2021-07-01
影响因子：
4.4
通讯作者：
Nichols JJ
中科院分区：
文献类型：
--
作者：
Khanal S;Bai Y;Ngo W;Nichols KK;Wilson L;Barnes S;Nichols JJ
调查先前的泪膜（PCTF）和梅博姆衍生的（O-酰基） - 富羟基 - 羟基脂肪酸（OAHFAS）和PCTF在梅博米亚腺体健康和功能障碍中稀疏之间的关联。
在195名合格的受试者中（18-84岁，女性62.6％），178名受试者和170名受试者提供了PCTF光学相干断层扫描（OCT）成像和质谱数据的泪液（n = 178）和Meibum（n = 170）使用超高分辨率的OCT在右眼中测量较薄的速率。在负离子模式下的三重质量光谱仪。 OAHFAS和PCTF稀疏率。
在Meibum和Tear样品中，分别检测到76个独特的OAHFAS。第一个PC载荷，六个OAHFA与PCTF稀疏率有负相关（18：2/16：2，ρ= -0.19，p = 0.01; 18：2/30：1，ρ= -0.21，p = 0.008; 18：1/28：1，ρ= -0.22，p = 0.004 ; 18：1/25：0，ρ= 0.22，p = 0 .006; 18：2/18：1，ρ= 0.48，p = 0.006电影衍生的Oahfas与PCTF稀疏率没有关联。
几个人类源自的OAHFA与PCTF稀疏显示了显着的关联，这表明这些OAHFA可以在PCTF的稳定和稀疏的基础机制中实现。
To investigate the association between precorneal tear film (PCTF)– and meibum-derived (O-Acyl)-omega-hydroxy fatty acids (OAHFAs) and PCTF thinning in meibomian gland health and dysfunction. Of 195 eligible subjects (18–84 years, 62.6% female), 178 and 170 subjects provided both PCTF optical coherence tomography (OCT) imaging and mass spectrometry data for tears (n = 178) and meibum (n = 170). The PCTF thinning rate was measured in the right eye using an ultra-high-resolution, custom-built OCT. Tear and meibum samples from the right eye were infused into the SCIEX 5600 TripleTOF mass spectrometer in the negative ion mode. Intensities (m/z) of preidentified OAHFAs were measured with Analyst 1.7TF and LipidView 1.3 (SCIEX). Principal component (PC) analyses and Spearman's correlations (ρ) were performed to evaluate the association between OAHFAs and PCTF thinning rates. In meibum and tear samples, 76 and 78 unique OAHFAs were detected, respectively. The first PC scores of the meibum-derived OAHFAs had statistically significant correlations with PCTF thinning rates (ρ = 0.18, P = 0.016). Among 10 OAHFAs with the highest first PC loadings, six OAHFAs had negative correlations with PCTF thinning rate (18:2/16:2, ρ = −0.19, P = 0.01; 18:2/30:1, ρ = −0.21, P = 0.008; 18:1/28:1, ρ = −0.22, P = 0.004; 18:1/30:1, ρ = −0.22, P = 0.005; 18:1/25:0, ρ = 0.22, P = 0 .006; and 18:1/26:1, ρ = −0.22, P = 0.006), while one OAHFA had a positive correlation with PCTF thinning rate (18:2/18:1, ρ = 0.48, P = 0.006). Tear film-derived OAHFAs had no association with the PCTF thinning rate. Several human meibum-derived OAHFAs showed significant associations with PCTF thinning, suggesting that these OAHFAs could be implicated in the mechanism underlying the stabilization and thinning of the PCTF. The tear-film derived OAHFAs were, however, independent of the rate of PCTF thinning.
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