ERF5 and ERF6 play redundant roles as positive regulators of JA/Et-mediated defense against Botrytis cinerea in Arabidopsis.
ERF5 and ERF6 play redundant roles as positive regulators of JA/Et-mediated defense against Botrytis cinerea in Arabidopsis.
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DOI：
10.1371/journal.pone.0035995
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Knight MR
中科院分区：
文献类型：
--
作者：
Moffat CS;Ingle RA;Wathugala DL;Saunders NJ;Knight H;Knight MR
拟南芥乙烯反应因子（ERF）家族由12个亚家族122个成员组成，但大多数成员的生物学功能尚不清楚。在第IX组ERF中，IXc亚组研究最多，包括ERF 1、ERF 14和HRA 59，它们在植物先天免疫中发挥作用。在这里，我们调查的生物学功能的两个成员较少研究IXb亚组：ERF 5和ERF 6。为了鉴定这些转录因子的潜在靶标，对组成型表达ERF 5或ERF 6的植物进行微阵列分析。ERF 5和ERF 6转基因植株中防御基因、JA/Et应答基因和GCC盒启动子基序基因的表达均显著上调，表明ERF 5和ERF 6可能作为JA介导的防御的正调控因子，并且它们的功能可能重叠。由于对坏死型病原体的防御通常是通过JA/Et-信号传导介导的，因此检查了对真菌坏死型灰葡萄孢的抗性。ERF 5或ERF 6的组成型表达导致显著增加的抗性。虽然对B的敏感性没有显著差异。尽管在erf 5或erf 6突变体中观察到灰霉病，但erf 5 erf 6双突变体显示出易感性的显著增加，这可能是由于受损的JA介导的基因表达，因为JA诱导的基因表达在双突变体中降低。综上所述，这些数据表明ERF 5和ERF 6在防御B中发挥积极但冗余的作用。灰叶由于JA/Et和水杨酸（SA）信号之间的相互拮抗作用是众所周知的，UV-C诱导的SA诱导基因，PR-1，进行了检查。ERF 5和ERF 6组成型过表达的诱导性降低与SA介导的信号转导的抑制一致，因为对无毒假单胞菌的敏感性增加。这些数据表明，ERF 5和ERF 6也可能在JA/Et和SA信号通路之间的拮抗性串扰中发挥作用。
The ethylene response factor (ERF) family in Arabidopsis thaliana comprises 122 members in 12 groups, yet the biological functions of the majority remain unknown. Of the group IX ERFs, the IXc subgroup has been studied the most, and includes ERF1, ERF14 and ORA59, which play roles in plant innate immunity. Here we investigate the biological functions of two members of the less studied IXb subgroup: ERF5 and ERF6. In order to identify potential targets of these transcription factors, microarray analyses were performed on plants constitutively expressing either ERF5 or ERF6. Expression of defense genes, JA/Et-responsive genes and genes containing the GCC box promoter motif were significantly upregulated in both ERF5 and ERF6 transgenic plants, suggesting that ERF5 and ERF6 may act as positive regulators of JA-mediated defense and potentially overlap in their function. Since defense against necrotrophic pathogens is generally mediated through JA/Et-signalling, resistance against the fungal necrotroph Botrytis cinerea was examined. Constitutive expression of ERF5 or ERF6 resulted in significantly increased resistance. Although no significant difference in susceptibility to B. cinerea was observed in either erf5 or erf6 mutants, the erf5 erf6 double mutant showed a significant increase in susceptibility, which was likely due to compromised JA-mediated gene expression, since JA-induced gene expression was reduced in the double mutant. Taken together these data suggest that ERF5 and ERF6 play positive but redundant roles in defense against B. cinerea. Since mutual antagonism between JA/Et and salicylic acid (SA) signalling is well known, the UV-C inducibility of an SA-inducible gene, PR-1, was examined. Reduced inducibilty in both ERF5 and ERF6 constitutive overexepressors was consistent with suppression of SA-mediated signalling, as was an increased susceptibility to avirulent Pseudomonas syringae. These data suggest that ERF5 and ERF6 may also play a role in the antagonistic crosstalk between the JA/Et and SA signalling pathways.
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