Ewe Vaginal Microbiota: Associations With Pregnancy Outcome and Changes During Gestation.
Ewe Vaginal Microbiota: Associations With Pregnancy Outcome and Changes During Gestation.
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DOI：
10.3389/fmicb.2021.745884
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发表时间：
2021
影响因子：
5.2
通讯作者：
Schmitz-Esser S
中科院分区：
文献类型：
--
作者：
Koester LR;Petry AL;Youngs CR;Schmitz-Esser S
繁殖性能对于专注于羊肉生产的畜牧生产企业的成功至关重要。生殖成功受到多种因素的影响，可能包括交配时和整个怀孕期间存在的生殖道微生物群落。很少有出版物能够鉴定牲畜的阴道微生物群落，而绵羊的阴道微生物群落就更少了。为了比较母羊阴道微生物群落，在配种前时间点 (S1) 和妊娠测试后 (S2) 从爱荷华州立大学养羊场的 67 只汉普郡和汉普郡 X 萨福克杂交母羊身上采集了阴道拭子。确定怀孕的动物在预期分娩后几天内再次取样（S3）。从这些拭子中提取 DNA，并进行 16S rRNA 基因 Illumina MiSeq 扩增子测序，以对系统中发现的细菌群落进行指纹识别。繁殖前时间点样本显示，后来发现怀孕或未怀孕的动物群落结构没有差异，但在第二个采样时间点，物种丰富度（Chao；P < 0.001）和物种多样性（Shannon；P < 0.001）发生了显着变化。 S2 时间点样本内较高的微生物多样性可能表明怀孕驱动的环境更加稳定，因为从 S2 到 S3 时间点，怀孕动物中保持了这种增加的多样性。此外，一些细菌系统型，如曼海姆菌、类似摇螺菌科的 OTU 和 Alistipes，在妊娠动物的 S1 或 S2 时间点更为丰富，这表明对妊娠结局有有益影响。这项研究确定了母羊阴道微生物群落在妊娠前和妊娠期间的变化，并就这些变化如何影响妊娠结局提供了推论。本文提供的信息提供了有关绵羊阴道微生物群落的新知识，并作为帮助指导研究人员未来提高绵羊繁殖性能的起点。
Reproductive performance is paramount to the success of livestock production enterprises focused on lamb meat production. Reproductive success is influenced by various factors, possibly including the reproductive tract microbial communities present at the time of copulation and throughout pregnancy. There are few publications that identify the vaginal microbial communities of livestock, and even fewer exist for sheep. To compare ewe vaginal microbial communities, vaginal swabs were taken from 67 Hampshire and Hampshire X Suffolk crossbred ewes from the Iowa State University sheep farm at a pre-breeding time point (S1) and after pregnancy testing (S2). Animals that were determined pregnant were sampled again within a few days of expected parturition (S3). DNA was extracted from these swabs, and 16S rRNA gene Illumina MiSeq amplicon sequencing was conducted to fingerprint the bacterial communities found within this system. Pre-breeding time point samples showed no differences in community structure between animals later found to be pregnant or non-pregnant, but significant changes were detected in species richness (Chao; P < 0.001) and species diversity (Shannon; P < 0.001) at the second sampling time point. A higher microbial diversity within the S2 time point samples may suggest a more stable environment driven by pregnancy, as this increased diversity is maintained in pregnant animals from the S2 to the S3 time point. Additionally, several bacterial phylotypes, such as Mannheimia, Oscillospiraceae-like OTUs and Alistipes, were more abundant at either the S1 or S2 time points in animals that established pregnancy, suggesting a beneficial effect on pregnancy outcome. This study identifies changes within the microbial communities of the ewe vagina before and during gestation and offers inferences on how these changes may impact pregnancy outcome. Information presented herein offers new knowledge about sheep vaginal microbial communities and serves as a starting point to help guide researchers to improve sheep reproductive performance in the future.
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影响因子：
1.4
作者：
Apprill, Amy;McNally, Sean;Weber, Laura
通讯作者：
Weber, Laura
影响因子：
1.4
作者：
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影响因子：
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作者：
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影响因子：
5.1
作者：
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通讯作者：
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影响因子：
3.5
作者：
Bicalho, M. L. S.;Machado, V. S.;Bicalho, R. C.
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