A direct tissue-grafting approach to increasing endogenous brown fat.
A direct tissue-grafting approach to increasing endogenous brown fat.
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DOI：
10.1038/s41598-018-25866-y
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发表时间：
2018-05-21
影响因子：
4.6
通讯作者：
Sia SK
中科院分区：
文献类型：
--
作者：
Blumenfeld NR;Kang HJ;Fenzl A;Song Z;Chung JJ;Singh R;Johnson R;Karakecili A;Feranil JB;Rossen NS;Zhang V;Jaggi S;McCarty B;Bessler S;Schwartz GJ;Grant R;Korner J;Kiefer FW;Gillette BM;Sia SK
有广泛的证据表明，通过白色脂肪组织（WAT）的布朗宁来增加棕色脂肪组织（BAT）的功能质量可以潜在地对抗肥胖和糖尿病。然而，大多数当前的方法集中于使患者暴露于高度不期望的副作用的药理学化合物的施用。在这里，我们描述了一种简单而直接的组织移植方法，通过体外布朗宁的皮下WAT，然后重新植入到主机增加BAT的质量，这种细胞治疗方法可能与现有的药理学方法协同作用。通过这种称为“exBAT”的方法，我们在小鼠和人体组织中鉴定了通过单一步骤将WAT的整个片段转化为BAT的条件，并且没有不必要的脱靶药理学作用。我们表明，离体，exBAT表现出UCP1免疫染色，脂滴形成，和线粒体代谢活性与天然BAT一致。在小鼠中，exBAT表现出高度持久的表型至少8周。总的来说，这些结果使一个简单的和可扩展的组织移植策略，而不是药理学的方法，增加内源性BAT和研究其对宿主体重和代谢的影响。
There is widespread evidence that increasing functional mass of brown adipose tissue (BAT) via browning of white adipose tissue (WAT) could potentially counter obesity and diabetes. However, most current approaches focus on administration of pharmacological compounds which expose patients to highly undesirable side effects. Here, we describe a simple and direct tissue-grafting approach to increase BAT mass through ex vivo browning of subcutaneous WAT, followed by re-implantation into the host; this cell-therapy approach could potentially act synergistically with existing pharmacological approaches. With this process, entitled “exBAT”, we identified conditions, in both mouse and human tissue, that convert whole fragments of WAT to BAT via a single step and without unwanted off-target pharmacological effects. We show that ex vivo, exBAT exhibited UCP1 immunostaining, lipid droplet formation, and mitochondrial metabolic activity consistent with native BAT. In mice, exBAT exhibited a highly durable phenotype for at least 8 weeks. Overall, these results enable a simple and scalable tissue-grafting strategy, rather than pharmacological approaches, for increasing endogenous BAT and studying its effect on host weight and metabolism.
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影响因子：
5.2
作者：
Boss O;Farmer SR
通讯作者：
Farmer SR
视网膜甲醛脱氢酶1调节白色脂肪组织中的热程序。
影响因子：
82.9
作者：
通讯作者：
--
DOI：
10.1097/med.0b013e328337a81f
发表时间：
2010-04
期刊：
Current opinion in endocrinology, diabetes, and obesity
影响因子：
--
作者：
Cypess AM;Kahn CR
通讯作者：
Kahn CR
影响因子：
2.9
作者：
Arterburn DE;Bogart A;Sherwood NE;Sidney S;Coleman KJ;Haneuse S;O'Connor PJ;Theis MK;Campos GM;McCulloch D;Selby J
通讯作者：
Selby J
影响因子：
29
作者：
Cypess AM;Weiner LS;Roberts-Toler C;Franquet Elía E;Kessler SH;Kahn PA;English J;Chatman K;Trauger SA;Doria A;Kolodny GM
通讯作者：
Kolodny GM