Comparative study of two types of herbal capsules with different Epimedium species for the prevention of ovariectomised-induced osteoporosis in rats.
Comparative study of two types of herbal capsules with different Epimedium species for the prevention of ovariectomised-induced osteoporosis in rats.
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DOI：
10.1016/j.jot.2015.07.001
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发表时间：
2016-01
影响因子：
6.6
通讯作者：
Qin L
中科院分区：
文献类型：
--
作者：
Chen SH;Wang XL;Zheng LZ;Dai Y;Zhang JY;Guo BL;Yang ZJ;Yao XS;Qin L
淫羊藿是中药仙灵骨葆胶囊中最重要的成骨药物。本研究比较了含有两种不同淫羊藿属（即，毛淫羊藿（XEP）或朝鲜淫羊藿（XEK），重点是化学成分和抗真菌功效。超高效液相色谱法用于证明不同的化学成分。使用已建立的卵巢切除（OVX）大鼠模型进行生物力学测试、组织学和细胞学评价，以表征和比较XEP和XEK之间的骨矿物质密度、骨强度、骨组织显微结构和生物活性。从XEK和XEP中鉴定出6种含量不同的黄酮类化合物。与OVX组相比，XEK组和XEP组的骨密度、弹性模量和抗压强度均显著较高（p < 0.05，n = 8）。组织形态计量学数据显示，两个治疗组的成骨细胞表面比率和类骨质面积显著较高，而侵蚀表面和脂肪细胞面积显著较低（p < 0.05，n = 6）。XEK或XEP与XLGB复方均对OVX所致的骨质疏松和骨力学性能下降有明显的预防作用。目前临床前实验研究的意义在于，用于配制XLGB胶囊的这两种淫羊藿属植物对预防雌激素耗竭诱导的骨质疏松症同样有效。
Epimedii Folium is the most important osteogenic herb formulated for the traditional Chinese Medicine Xian Ling Gu Bao (XLGB) capsule. The present study compared XLGB capsules containing two different Epimedium species, i.e., either Epimedium pubescens (XEP) or Epimedium koreanum (XEK), with the focus being on the chemical constituents and antiosteoporotic efficacy. Ultra performance liquid chromatography was used to demonstrate the different chemical constituents. Biomechanical tests, histological, and cytological evaluation were performed to characterise and compare the bone mineral density, bone strength, microstructure of bone tissue, and biological activity between XEP and XEK using an established ovariectomised (OVX) rat model. Six flavonoids with different contents between XEK and XEP were identified. As compared with the OVX group, significantly higher bone mineral density, elastic-modulus, and compressive strength were found in both the XEK group and XEP group (p < 0.05 for all, n = 8). Histomorphometric data presented significantly higher osteoblast surface ratio and osteoid area accompanied by significantly lower values of erosion surface and adiopocytes area in two treatment groups (p < 0.05, n = 6). XLGB Fufang with either XEK or XEP all showed significant preventive effects in OVX-induced osteoporosis and deterioration of bone mechanical properties. The significance of the current preclinical experimental study was that these two Epimedium species used for formulating XLGB capsules were equally effective for the prevention of oestrogen-depletion induced osteoporosis.
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影响因子：
2.8
作者：
Chen H;Zhou X;Fujita H;Onozuka M;Kubo KY
通讯作者：
Kubo KY
DOI：
10.1016/j.jpba.2014.03.024
发表时间：
2014-08-05
影响因子：
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作者：
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作者：
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影响因子：
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作者：
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影响因子：
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作者：
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