Bacterial inactivation by a singlet oxygen bubbler: identifying factors controlling the toxicity of (1)O2 bubbles.
Bacterial inactivation by a singlet oxygen bubbler: identifying factors controlling the toxicity of (1)O2 bubbles.
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DOI：
10.1021/es303645n
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发表时间：
2012-11-06
影响因子：
11.4
通讯作者：
Greer, Alexander
中科院分区：
文献类型：
--
作者：
Bartusik, Dorota;Aebisher, David;Lyons, Alan M.;Greer, Alexander
开发了一种微型光反应器装置，以产生含有对细菌和真菌有毒的单线态氧的气泡（尺寸：1.4 mm直径，90 μL）。由于单线态氧迅速衰变为三线态氧，气泡除了O2之外不会留下任何废物或副产品。从脱气、空气饱和和充氧溶液中的比较研究，推断单线态氧气泡主要通过气泡内部和外部的氧梯度来杀灭大肠杆菌和烟曲霉，使得单线态氧被溶剂化并扩散通过水溶液，直到其与目标生物体反应。因此，单线态氧泡毒性与溶液中溶解氧的量成反比。在第二种机制中，单线态氧直接与E相互作用。大肠杆菌聚集在气液界面，尽管这种机制的运行速度大约慢10倍。由于封装在气泡的气体核心和0.98 ms的寿命，气泡可以穿过相对较长的0.39 mm距离，携带1 O2进入溶液;相比之下，完全溶剂化的1 O2在H2O中的扩散距离要短得多（~150 nm）。到达外部空气/水界面的气泡不含1 O2。通过添加去污剂分子和Ca ~（2+）离子，探讨了1O_2对生物体的灭活机理。结果表明，E.在气泡的空气-水界面处的大肠杆菌导致含1 O2的气泡的毒性增强。单线态氧装置提供了有趣的可能性，创造基于光动力（1 O2）气泡载体的新型消毒策略。
A microphotoreactor device was developed to generate bubbles (sized: 1.4 mm diameter, 90 μL) containing singlet oxygen at levels toxic to bacteria and fungus. As singlet oxygen decays rapidly to triplet oxygen, the bubbles leave behind no waste or by-products other than O2. From a comparative study in deaerated, air saturated, and oxygenated solutions, it was reasoned that the singlet oxygen bubbles inactivate Escherichia coli and Aspergillus fumigatus, mainly by an oxygen gradient inside and outside of the bubble such that singlet oxygen is solvated and diffuses through the aqueous solution until it reacts with the target organism. Thus, singlet oxygen bubble toxicity was inversely proportional to the amount of dissolved oxygen in solution. In a second mechanism, singlet oxygen interacts directly with E. coli that accumulate at the gas-liquid interface although this mechanism operates at a rate approximately 10 times slower. Due to encapsulation in the gaseous core of the bubble and a 0.98 ms lifetime, the bubbles can traverse relatively long 0.39 mm distances carrying 1O2 far into the solution; by comparison the diffusion distance of 1O2 fully solvated in H2O is much shorter (~150 nm). Bubbles that reached the outer air/water interface contained no 1O2. The mechanism by which 1O2 deactivated organisms was explored through the addition of detergent molecules and Ca2+ ions. Results indicate that the preferential accumulation of E. coli at the air-water interface of the bubble leads to enhanced toxicity of bubbles containing 1O2. The singlet oxygen device offers intriguing possibilities for creating new types of disinfection strategies based on photodynamic (1O2) bubble carriers.
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影响因子：
15.9
作者：
DRUMM, B;NEUMANN, AW;SHERMAN, PM
通讯作者：
SHERMAN, PM
影响因子：
22.1
作者：
Villen, Laura;Manjon, Francisco;Orellana, Guillermo
通讯作者：
Orellana, Guillermo
影响因子：
3.9
作者：
Zawala, Jan;Malysa, Kazimierz
通讯作者：
Malysa, Kazimierz
影响因子：
11.4
作者：
Manjon, Francisco;Villen, Laura;Orellana, Guillermo
通讯作者：
Orellana, Guillermo
影响因子：
3.3
作者：
DAHL, TA;MIDDEN, WR;HARTMAN, PE
通讯作者：
HARTMAN, PE