3-D reconstruction of rice leaf tissue for proper estimation of surface area of mesophyll cells and chloroplasts facing intercellular airspaces from 2-D section images.
3-D reconstruction of rice leaf tissue for proper estimation of surface area of mesophyll cells and chloroplasts facing intercellular airspaces from 2-D section images.
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DOI：
10.1093/aob/mcac133
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发表时间：
2022-12-31
期刊：
影响因子：
4.2
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中科院分区：
文献类型：
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叶肉细胞（\(S_{mes}\)）和叶绿体（\(S_{c}\)）面向细胞间隙（\(IAS\)）的表面积是从叶片解剖结构估算光合活性的重要参数。尽管\(S_{mes}\)和\(S_{c}\)是基于叶肉细胞（\(MCs\)）的形状假设来估算的，但对于具有凹凸表面的水稻叶肉细胞，该假设是否正确值得怀疑。因此，在本研究中，我们建立了一种水稻叶片组织中\(IAS\)的三维表征重建方法，以便利用三维图像计算实际的\(S_{mes}\)和\(S_{c}\)，并确定基于二维切片图像估算\(S_{mes}\)和\(S_{c}\)的正确形状假设。
我们利用连续切片光学显微镜对水稻叶片组织中的\(IAS\)、\(MCs\)和叶绿体进行三维表征重建。将从三维表征中获得的实际\(S_{mes}\)和\(S_{c}\)值与从二维图像估算的值进行比较，以便利用同一叶片样本在不同方向（纵切面和横切面）找到正确的形状特异性假设（扁球体或长球体）。
三维表征方法显示，\(IAS\)和\(MCs\)的体积分别占去除表皮的水稻叶片组织的\(30\%\)和\(70\%\)，叶绿体的体积占\(MCs\)的\(44\%\)。切片方向的形状特异性假设影响了利用二维切片图像对\(S_{mes}\)和\(S_{c}\)的估算，差异为\(10 - 38\%\)。
利用连续切片光学显微镜成功地重建了水稻叶片组织的三维表征，结果表明，将\(MCs\)假设为扁球体的纵切面比将\(MCs\)假设为长球体的横切面更能准确地估算水稻叶片的\(S_{mes}\)和\(S_{c}\)。
The surface area of mesophyll cells (Smes) and chloroplasts (Sc) facing the intercellular airspace (IAS) are important parameters for estimating photosynthetic activity from leaf anatomy. Although Smes and Sc are estimated based on the shape assumption of mesophyll cells (MCs), it is questionable if the assumption is correct for rice MCs with concave–convex surfaces. Therefore, in this study, we establish a reconstruction method for the 3-D representation of the IAS in rice leaf tissue to calculate the actual Smes and Sc with 3-D images and to determine the correct shape assumption for the estimation of Smes and Sc based on 2-D section images. We used serial section light microscopy to reconstruct 3-D representations of the IAS, MCs and chloroplasts in rice leaf tissue. Actual Smes and Sc values obtained from the 3-D representation were compared with those estimated from the 2-D images to find the correct shape-specific assumption (oblate or prolate spheroid) in different orientations (longitudinal and transverse sections) using the same leaf sample. The 3-D representation method revealed that volumes of the IAS and MCs accounted for 30 and 70 % of rice leaf tissue excluding epidermis, respectively, and the volume of chloroplasts accounted for 44 % of MCs. The shape-specific assumption on the sectioning orientation affected the estimation of Smes and Sc using 2-D section images with discrepancies of 10–38 %. The 3-D representation of rice leaf tissue was successfully reconstructed using serial section light microscopy and suggested that estimation of Smes and Sc of the rice leaf is more accurate using longitudinal sections with MCs assumed as oblate spheroids than using transverse sections with MCs as prolate spheroids.
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DOI：
10.1111/tpj.13727
发表时间：
2017-12
期刊：
The Plant journal : for cell and molecular biology
影响因子：
--
作者：
Lehmeier C;Pajor R;Lundgren MR;Mathers A;Sloan J;Bauch M;Mitchell A;Bellasio C;Green A;Bouyer D;Schnittger A;Sturrock C;Osborne CP;Rolfe S;Mooney S;Fleming AJ
通讯作者：
Fleming AJ
影响因子：
1
作者：
Psaras, GK;Diamantopoulos, GS;Makrypoulias, CP
通讯作者：
Makrypoulias, CP
影响因子：
7.3
作者：
Scafaro, Andrew P.;Von Caemmerer, Susanne;Atwell, Brian J.
通讯作者：
Atwell, Brian J.
影响因子：
6.9
作者：
THAIN, JF
通讯作者：
THAIN, JF
影响因子：
4.2
作者：
Oi, Takao;Enomoto, Sakiko;Taniguchi, Mitsutaka
通讯作者：
Taniguchi, Mitsutaka