A novel genetic engineering platform for the effective management of biological contaminants for the production of microalgae.
A novel genetic engineering platform for the effective management of biological contaminants for the production of microalgae.
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DOI：
10.1111/pbi.12564
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发表时间：
2016-10
影响因子：
13.8
通讯作者：
Herrera-Estrella L
中科院分区：
文献类型：
--
作者：
Loera-Quezada MM;Leyva-González MA;Velázquez-Juárez G;Sanchez-Calderón L;Do Nascimento M;López-Arredondo D;Herrera-Estrella L
利用太阳能的微藻培养是生物技术生产生物燃料和一系列高价值产品（包括药品，肥料和饲料）的最具成本效益的系统之一。然而，微藻培养的主要限制之一是潜在的生物污染物污染，例如细菌、真菌、浮游动物或其他不需要的微藻。在封闭式生物反应器中，污染控制需要对培养基、容器和所有材料进行灭菌，这增加了生产成本，而开放式池塘系统严重限制了在极端环境条件下可以培养以防止污染的物种数量。在这里，我们报告的代谢工程衣原体reinhardtii使用亚磷酸盐作为其唯一的磷源，通过表达ptxD基因从假单胞菌stutzeri WM 88，它编码的亚磷酸盐氧化还原酶能够氧化成磷酸盐使用NAD作为辅因子。当用亚磷酸盐作为唯一磷源施肥时，工程化的莱茵衣藻品系能够成为混合培养物中的优势物种。我们的研究结果代表了微藻生产的新平台，对于封闭的光生物反应器和开放的池塘系统都可能有用，而不需要使用无菌条件，也不需要抗生素或除草剂来防止生物污染物的污染。
Microalgal cultivation that takes advantage of solar energy is one of the most cost‐effective systems for the biotechnological production of biofuels, and a range of high value products, including pharmaceuticals, fertilizers and feed. However, one of the main constraints for the cultivation of microalgae is the potential contamination with biological pollutants, such as bacteria, fungi, zooplankton or other undesirable microalgae. In closed bioreactors, the control of contamination requires the sterilization of the media, containers and all materials, which increases the cost of production, whereas open pond systems severely limits the number of species that can be cultivated under extreme environmental conditions to prevent contaminations. Here, we report the metabolic engineering of Chlamydomonas reinhardtii to use phosphite as its sole phosphorus source by expressing the ptxD gene from Pseudomonas stutzeri WM88, which encodes a phosphite oxidoreductase able to oxidize phosphite into phosphate using NAD as a cofactor. Engineered C. reinhardtii lines are capable of becoming the dominant species in a mixed culture when fertilized with phosphite as a sole phosphorus source. Our results represent a new platform for the production of microalgae, potentially useful for both closed photobioreactors and open pond systems without the need for using sterile conditions nor antibiotics or herbicides to prevent contamination with biological pollutants.
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影响因子：
4.1
作者：
Kazamia, Elena;Aldridge, David C.;Smith, Alison G.
通讯作者：
Smith, Alison G.
影响因子：
3.7
作者：
Letcher PM;Lopez S;Schmieder R;Lee PA;Behnke C;Powell MJ;McBride RC
通讯作者：
McBride RC
影响因子：
6.9
作者：
Berkowitz O;Jost R;Kollehn DO;Fenske R;Finnegan PM;O'Brien PA;Hardy GE;Lambers H
通讯作者：
Lambers H
影响因子：
46.9
作者：
Lizbeth Lopez-Arredondo, Damar;Herrera-Estrella, Luis
通讯作者：
Herrera-Estrella, Luis
影响因子：
5.2
作者：
Loera-Quezada, Maribel M.;Leyva-Gonzalez, Marco Antonio;Herrera-Estrella, Luis
通讯作者：
Herrera-Estrella, Luis