Role of IME1 expression in regulation of meiosis in Saccharomyces cerevisiae
Role of IME1 expression in regulation of meiosis in Saccharomyces cerevisiae
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IME1 表达在酿酒酵母减数分裂调节中的作用
DOI：
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发表时间：
1990
影响因子：
5.3
通讯作者：
A. Mitchell
中科院分区：
文献类型：
--
作者：
Harold E. Smith;SU SOPHIAS.Y.;L. Neigeborn;Suzanne E. Driscoll;A. Mitchell
在酿酒酵母中，减数分裂和孢子形成需要两种信号：饥饿和MAT产物α 1和α 2，它们决定了α/α细胞类型。这些信号导致IME 1（减数分裂诱导物）基因的表达增加，这是孢子形成和孢子形成特异性基因表达所需的。我们在这里报告的序列的IME 1基因和IME 1表达的后果从GAL 1启动子。推导的IME 1产物是一个360个氨基酸的蛋白质，具有富含酪氨酸的C-末端区域。PGAL 1-IME 1在营养a/alpha细胞中的表达导致四种早期孢子形成特异性转录物（IME 2，SPO 11，SPO 13和HOP 1）的中度积累;饥饿后转录物的积累增加了3至10倍。两个孢子形成特异性转录本（SPS 1和SPS 2）正常表达后，没有积累，直到PGAL 1-IME 1菌株饥饿，完整的IME 1基因没有激活PGAL 1-IME 1的表达。在缺乏α 2或α 1的α或α细胞中，PGAL 1-IME 1的表达导致与α/α细胞中相同的IME 2和SPO 13表达模式，如ime 2：：lacZ和spo 13：：lacZ融合所测量的。因此，在野生型菌株中，饥饿的α/α细胞中IME 1表达的增加可以完全解释早期孢子形成特异性基因表达的细胞类型控制，但仅部分解释营养控制。PGAL 1-IME 1表达不会导致生长细胞形成孢子，但允许氨基酸限制性细胞有效形成孢子，否则孢子形成较差。我们认为IME 1主要作为早期孢子形成特异性基因的正调控因子，并且生长停滞是执行孢子形成程序的独立先决条件。
Two signals are required for meiosis and spore formation in the yeast Saccharomyces cerevisiae: starvation and the MAT products a1 and alpha 2, which determine the a/alpha cell type. These signals lead to increased expression of the IME1 (inducer of meiosis) gene, which is required for sporulation and sporulation-specific gene expression. We report here the sequence of the IME1 gene and the consequences of IME1 expression from the GAL1 promoter. The deduced IME1 product is a 360-amino-acid protein with a tyrosine-rich C-terminal region. Expression of PGAL1-IME1 in vegetative a/alpha cells led to moderate accumulation of four early sporulation-specific transcripts (IME2, SPO11, SPO13, and HOP1); the transcripts accumulated 3- to 10-fold more after starvation. Two sporulation-specific transcripts normally expressed later (SPS1 and SPS2) did not accumulate until PGAL1-IME1 strains were starved, and the intact IME1 gene was not activated by PGAL1-IME1 expression. In a or alpha cells, which lack alpha 2 or a1, expression of PGAL1-IME1 led to the same pattern of IME2 and SPO13 expression as in a/alpha cells, as measured with ime2::lacZ and spo13::lacZ fusions. Thus, in wild-type strains, the increased expression of IME1 in starved a/alpha cells can account entirely for cell type control, but only partially for nutritional control, of early sporulation-specific gene expression. PGAL1-IME1 expression did not cause growing cells to sporulate but permitted efficient sporulation of amino acid-limited cells, which otherwise sporulated poorly. We suggest that IME1 acts primarily as a positive regulator of early sporulation-specific genes and that growth arrest is an independent prerequisite for execution of the sporulation program.
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鉴定控制酵母中早期减数分裂基因表达的负调控基因。
DOI：
10.1073/pnas.86.24.10018
发表时间：
1989
影响因子：
11.1
作者：
Strich,R;Slater,MR;Esposito,RE
通讯作者：
Esposito,RE
DOI：
10.1073/pnas.84.22.8035
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1987-11-01
影响因子：
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作者：
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影响因子：
5.6
作者：
PASSMORE, S;MAINE, GT;TYE, BK
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TYE, BK
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影响因子：
3.5
作者：
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影响因子：
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作者：
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