Ontogeny of aromatic L-amino acid decarboxylase-containing tubule cells in rat kidney.
Ontogeny of aromatic L-amino acid decarboxylase-containing tubule cells in rat kidney.
复制标题
大鼠肾中含有芳香族L-氨基酸脱羧酶的小管细胞的个体发育。
DOI：
10.1038/ki.1992.326
复制
发表时间：
1992
影响因子：
19.6
通讯作者：
Hökfelt,T
中科院分区：
文献类型：
--
作者：
Meister,B;Fried,G;Holgert,H;Aperia,A;Hökfelt,T
大鼠肾脏含芳香族L氨基酸脱羧酶的小管细胞的个体发育。多巴胺在调节肾脏钠转运中起着重要作用。近端小管细胞通过芳香族L氨基酸脱羧酶(AADC)对L多巴进行脱羧化，从而产生多巴胺。用间接免疫荧光和原位杂交组织化学方法检测了AADC样免疫反应(-LI)和AADC mRNA在大鼠肾脏发育过程中的存在和细胞定位。孕18天仅见少量散在的AADC免疫反应(-IR)小管细胞凝聚成管腔。自孕21天起，内侧皮质可见大量AADC-IR小管细胞，而外侧皮质缺乏AADC-LI。出生后24小时内，内侧皮质的AADC-IR细胞可鉴定为近端小管细胞。在第3天和第5天，内侧皮质AADC-IR近端小管细胞数量增加，而外侧皮质AADC-IR阴性细胞数量较少。第8天开始，外侧皮质可见AADC-IR细胞。AADC免疫荧光强度在40d时明显降低，80d时几乎没有AADC-LI。肾内多巴胺水平在3~20天呈上升趋势，在20~40天和40~80天呈显著下降趋势。出生后18h，肾脏未检测到AADC基因的表达，6d时，肾脏内侧皮质可检测到AADC基因的表达。12、19和40d时，AADC基因在整个皮质均可见表达。结果表明，大鼠肾脏近端小管在出生前即可形成多巴胺，出生后1个月达高峰。
Ontogeny of aromatic L-amino acid decarboxylase-containing tubule cells in rat kidney. Dopamine plays an important role in regulation of renal sodium transport. Proximal tubule cells produce dopamine after decarboxylation of L-DOPA via the enzyme aromatic L-amino acid decarboxylase (AADC). The presence and cellular localization of AADC-like immunoreactivity (-LI) and AADC mRNA were examined during pre- and postnatal development in rat kidney by indirect immunofluorescence and in situ hybridization histochemistry. Few scattered condensations of AADC-immunoreactive (-IR) tubule cells forming a lumen were detected on gestational day 18. From gestational day 21, many AADC-IR tubule cells were observed in the inner cortex, whereas the outer cortex lacked AADC-LI. Within 24 hours of birth, AADC-IR cells in the inner cortex could be identified as proximal tubule cells. During day 3 and 5 there was an increase in number of AADC-IR proximal tubule cells in the inner cortex, leaving less amount of AADC-negative cells in the outer cortex. Starting from day 8, AADC-IR cells could be seen in the outer cortex. An apparent decrease in AADC-immunofluorescence intensity was observed at 40 days, and at 80 days virtually no AADC-LI could be demonstrated. Intrarenal levels of dopamine showed a tendency to increase between 3 and 20 days, and showed significant decreases between 20 to 40 days and between 40 to 80 days. AADC mRNA was not detected in the kidney at 18 hours after birth, but could be observed in the inner cortex at 6 days. At 12, 19 and 40 days AADC mRNA was seen in the entire cortex. The results indicate that dopamine can be formed prenatally in the proximal tubules of the rat kidney and that the levels of dopamine increase postnatally to reach a peak at one month.
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