Transcriptomics, metabolomics and histology indicate that high-carbohydrate diet negatively affects the liver health of blunt snout bream (Megalobrama amblycephala).
Transcriptomics, metabolomics and histology indicate that high-carbohydrate diet negatively affects the liver health of blunt snout bream (Megalobrama amblycephala).
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DOI：
10.1186/s12864-017-4246-9
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发表时间：
2017-11-09
期刊：
影响因子：
4.4
通讯作者：
Wang WM
中科院分区：
文献类型：
--
作者：
Prisingkorn W;Prathomya P;Jakovlić I;Liu H;Zhao YH;Wang WM
在水产动物饲料生产中引入大量相对廉价的碳水化合物以减少昂贵蛋白质的全球趋势影响了经济上重要的鲤科鱼类--团头鱼(Megalobrama Amblycephala)的水产养殖。这种饮食变化导致新陈代谢紊乱的患病率增加，经常造成经济损失。与肥胖相关的高碳水化合物饮食摄入是人类非酒精性脂肪性肝病(NAFLD)的主要原因之一。我们进行了为期八周的饲养试验，以更好地了解高碳水化合物饮食(HCBD)如何影响这种鱼的肝脏健康。肝体指数和血脂含量明显高于对照组(P<0.05)。组织学结果还提示肝细胞癌组肝脏出现病理改变，脂质过度积聚，有肝损害的征象。代谢组学和血清生化分析显示，HCBD组肝脏损害的一些代谢物增加。这组人的甜菜碱水平也很低，甜菜碱是维持健康肝功能的关键代谢物。转录和定量聚合酶链式反应分析表明，HCBD对NAFLD和胰岛素信号通路相关的大量基因的表达有强烈的影响，这可能导致肝细胞胰岛素抵抗的发展，肝脏的病理变化，最终导致NAFLD。转录学、代谢组学和组织学结果都表明肝脏损伤的早期症状。然而，这些是否真的会导致NAFLD在更长的一段时间后发展，仍然没有定论。此外，在与几种神经退行性疾病相关的HCBD组中，非常大量的上调基因是由高碳水化合物饮食导致的神经退行性变化的强烈迹象。这表明，鱼类可能是研究与高碳水化合物饮食相关的神经退行性变化的一个很好的模型。本文的在线版本(10.1186/s12864-0174246-9)包含补充材料，可供授权用户使用。
Global trend of the introduction of high levels of relatively cheap carbohydrates to reduce the amount of costly protein in the aquatic animal feed production has affected the aquaculture of an economically important cyprinid fish, blunt snout bream (Megalobrama amblycephala). This dietary shift has resulted in increased prevalence of metabolic disorders, often causing economic losses. High dietary intake of carbohydrates, associated with obesity, is one of the major causes of non-alcoholic fatty liver disease (NAFLD) in humans. We have conducted an eight-week feeding trial to better understand how a high-carbohydrate diet (HCBD) affects the liver health in this fish. Hepatosomatic index and lipid content were significantly (P < 0.05) higher in the HCBD group. Histology results also suggested pathological changes in the livers of HCBD group, with excessive lipid accumulation and indication of liver damage. Metabolomics and serum biochemistry analyses showed that a number of metabolites indicative of liver damage were increased in the HCBD group. This group also exhibited low levels of betaine, which is a metabolite crucial for maintaining the healthy liver functions. Transcriptomic and qPCR analyses indicated that HCBD had a strong impact on the expression of a large number of genes associated with the NAFLD and insulin signalling pathways, which may lead to the development of insulin resistance in hepatocytes, pathological liver changes, and eventually the NAFLD. Transcriptomics, metabolomics and histology results all indicate early symptoms of liver damage. However whether these would actually lead to the development of NAFLD after a longer period of time, remains inconclusive. Additionally, a very high number of upregulated genes in the HCBD group associated with several neurodegenerative diseases is a strong indication of neurodegenerative changes caused by the high-carbohydrate diet in blunt snout bream. This suggests that fish might present a good model to study neurodegenerative changes associated with high-carbohydrate diet in humans. The online version of this article (10.1186/s12864-017-4246-9) contains supplementary material, which is available to authorized users.
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DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y
DOI：
10.1016/j.cbpa.2006.05.002
发表时间：
2006-09-01
影响因子：
2.3
作者：
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Oliva-Teles, A.
影响因子：
25.7
作者：
Bergheim, Ina;Weber, Synia;Bischoff, Stephan C.
通讯作者：
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影响因子：
5.8
作者：
Conesa, A;Götz, S;Robles, M
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Robles, M
影响因子：
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作者：
Enes, P.;Panserat, S.;Oliva-Teles, A.
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Oliva-Teles, A.