ycf1, the most promising plastid DNA barcode of land plants.
ycf1, the most promising plastid DNA barcode of land plants.
复制标题
DOI：
10.1038/srep08348
复制
发表时间：
2015-02-12
影响因子：
4.6
通讯作者：
Zhou S
中科院分区：
文献类型：
--
作者：
Dong W;Xu C;Li C;Sun J;Zuo Y;Shi S;Cheng T;Guo J;Zhou S
DNA条形码是用于识别物种的DNA片段。对于陆生植物，质体基因组的DNA片段可能是主要考虑因素。不幸的是，大多数质体候选条形码缺乏物种水平的分辨率。在物种水平上鉴定高分辨率的DNA条形码是植物DNA条形码成功的关键。我们搜索了可用的质体基因组中最可变的区域，并使用大量的树种和七个采样良好的植物组测试了最佳候选人。质体基因ycf 1的两个区域ycf 1a和ycf 1b是最可变的基因座，比现有的质体候选条形码更好，可以作为陆地植物的条形码。设计引物扩增这些区域，这些引物的PCR成功率为82.80%至98.17%。在420个树种中，ycf 1b能区分357个树种，比matK和rbcL的组合略好。对于采样良好的代表性植物组，ycf 1b通常比matK，rbcL和trnH-psbA中的任何一个都表现得更好。我们的结论是，ycf 1a或ycf 1b是最可变的质体基因组区域，可以作为陆地植物的核心条形码。
A DNA barcode is a DNA fragment used to identify species. For land plants, DNA fragments of plastid genome could be the primary consideration. Unfortunately, most of the plastid candidate barcodes lack species-level resolution. The identification of DNA barcodes of high resolution at species level is critical to the success of DNA barcoding in plants. We searched the available plastid genomes for the most variable regions and tested the best candidates using both a large number of tree species and seven well-sampled plant groups. Two regions of the plastid gene ycf1, ycf1a and ycf1b, were the most variable loci that were better than existing plastid candidate barcodes and can serve as a barcode of land plants. Primers were designed for the amplification of these regions, and the PCR success of these primers ranged from 82.80% to 98.17%. Of 420 tree species, 357 species could be distinguished using ycf1b, which was slightly better than the combination of matK and rbcL. For the well-sampled representative plant groups, ycf1b generally performed better than any of the matK, rbcL and trnH-psbA. We concluded that ycf1a or ycf1b is the most variable plastid genome region and can serve as a core barcode of land plants.
登录
查看更多内容
DOI：
10.1098/rsbl.2003.0025
发表时间：
2003-08-07
影响因子：
4.7
作者：
Hebert, PDN;Ratnasingham, S;deWaard, JR
通讯作者：
deWaard, JR
影响因子：
1
作者：
Gernandt, David S.;Hernandez-Leon, Sergio;Perez de la Rosa, Jorge A.
通讯作者：
Perez de la Rosa, Jorge A.
影响因子：
3.7
作者：
Little DP;Knopf P;Schulz C
通讯作者：
Schulz C
影响因子：
10.7
作者：
Goremykin, VV;Holland, B;Hellwig, FH
通讯作者：
Hellwig, FH
影响因子：
3.7
作者：
Chen S;Yao H;Han J;Liu C;Song J;Shi L;Zhu Y;Ma X;Gao T;Pang X;Luo K;Li Y;Li X;Jia X;Lin Y;Leon C
通讯作者：
Leon C