Validation of the ITS2 region as a novel DNA barcode for identifying medicinal plant species.
Validation of the ITS2 region as a novel DNA barcode for identifying medicinal plant species.
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DOI：
10.1371/journal.pone.0008613
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发表时间：
2010-01-07
期刊：
影响因子：
3.7
通讯作者：
Leon C
中科院分区：
文献类型：
--
作者：
Chen S;Yao H;Han J;Liu C;Song J;Shi L;Zhu Y;Ma X;Gao T;Pang X;Luo K;Li Y;Li X;Jia X;Lin Y;Leon C
生命条形码联盟的植物工作组推荐rbcL + matK的双位点组合作为植物条形码，但该组合已被证明在物种水平上成功区分了来自550个物种的907个样本，概率为72%。该小组承认，由于识别率低，双位点条形码还远未完美，搜索还没有结束。在这里，我们比较了7个候选DNA条形码（psbA-trnH，matK，rbcL，rpoC 1，ycf 5，ITS 2和ITS）从药用植物物种。我们的排名标准包括PCR扩增效率，差异内和种间的分歧，和DNA条形码差距。我们的数据表明，核核糖体DNA的第二个内转录间隔区（ITS 2）代表了最适合的DNA条形码应用的区域。此外，我们测试了ITS 2的识别能力，超过6600植物样品属于4800种，来自753个不同的属，发现与ITS 2的成功识别率为92.7%，在物种水平。ITS 2区域可作为标准DNA条形码用于药用植物及其近缘物种的鉴定。我们还建议，ITS 2可以作为一种新的通用条形码识别范围更广的植物类群。
The plant working group of the Consortium for the Barcode of Life recommended the two-locus combination of rbcL + matK as the plant barcode, yet the combination was shown to successfully discriminate among 907 samples from 550 species at the species level with a probability of 72%. The group admits that the two-locus barcode is far from perfect due to the low identification rate, and the search is not over. Here, we compared seven candidate DNA barcodes (psbA-trnH, matK, rbcL, rpoC1, ycf5, ITS2, and ITS) from medicinal plant species. Our ranking criteria included PCR amplification efficiency, differential intra- and inter-specific divergences, and the DNA barcoding gap. Our data suggest that the second internal transcribed spacer (ITS2) of nuclear ribosomal DNA represents the most suitable region for DNA barcoding applications. Furthermore, we tested the discrimination ability of ITS2 in more than 6600 plant samples belonging to 4800 species from 753 distinct genera and found that the rate of successful identification with the ITS2 was 92.7% at the species level. The ITS2 region can be potentially used as a standard DNA barcode to identify medicinal plants and their closely related species. We also propose that ITS2 can serve as a novel universal barcode for the identification of a broader range of plant taxa.
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DOI：
10.1098/rsbl.2003.0025
发表时间：
2003-08-07
影响因子：
4.7
作者：
Hebert, PDN;Ratnasingham, S;deWaard, JR
通讯作者：
deWaard, JR
评估序列长度对真菌DNA条形码的影响。
DOI：
10.1111/j.1471-8286.2007.01698.x
发表时间：
2007-05-01
期刊：
Molecular ecology notes
影响因子：
--
作者：
Min XJ;Hickey DA
通讯作者：
Hickey DA
DOI：
10.1073/pnas.0503123102
发表时间：
2005-06-07
影响因子：
11.1
作者：
Kress, WJ;Wurdack, KJ;Janzen, DH
通讯作者：
Janzen, DH
DOI：
10.1073/pnas.0812297106
发表时间：
2009-02-24
影响因子：
11.1
作者：
Awano, Tomoyuki;Johnson, Gary S.;Coates, Joan R.
通讯作者：
Coates, Joan R.
DNA 条形码：基于全面采样的错误率。
影响因子：
9.8
作者：
Meyer CP;Paulay G
通讯作者：
Paulay G