The bone regenerative capacity of canine mesenchymal stem cells is regulated by site-specific multilineage differentiation.
The bone regenerative capacity of canine mesenchymal stem cells is regulated by site-specific multilineage differentiation.
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DOI：
10.1016/j.oooo.2016.09.011
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发表时间：
2017-03
期刊：
影响因子：
--
通讯作者：
Akintoye SO
中科院分区：
文献类型：
--
作者：
Bugueño J;Li W;Salat P;Qin L;Akintoye SO
间充质干细胞（MSCs）是一种具有多能性的牙科治疗方法。选择供体间充质干细胞至关重要，因为临床前研究中常用的比格犬（犬）已显示出不同的结果，并且尚不清楚犬间充质干细胞（cMSC）是否具有骨骼部位特异性。本研究测试了颌骨和长骨cMSCs是否具有不同的体外和体内多谱系分化能力。从四只健康比格犬的下颌骨（M-cMSC）和股骨（F-cMSC）分离原代cMSC。股骨作为非经口对照。评估克隆形成和增殖能力。体外成骨、成软骨、成脂和神经多系分化与体内骨再生和临床应用潜力相关。相对于F-cMSC，M-cMSC显示出克隆形成和增殖能力的两倍增加（p =0.006）。基于碱性磷酸酶（p =0.04）、骨唾液蛋白（p =0.05）和骨钙素（p =0.03）的体外成骨以及脂肪生成（p =0.007）和软骨生成（p =0.009）的M-cMSC相对较高，并且与增强的M-cMSC骨再生能力相关。神经表达标志物nestin和β III-tubulin无显著差异。下颌骨间充质干细胞的分化能力和骨再生能力的增强可能使其成为一种良好的下颌骨位点特异性再生的供体移植材料。
Mesenchymal stem cells (MSCs) are promising therapies in dentistry due to their multipotent properties. Selecting donor MSCs is crucial because beagle dogs (canines) commonly used in pre-clinical studies have shown variable outcomes and it is unclear whether canine MSCs (cMSCs) are skeletal site-specific. This study tested whether jaw and long bone cMSCs have disparate in vitro and in vivo multilineage differentiation capabilities. Primary cMSCs were isolated from mandible (M-cMSCs) and femur (F-cMSCs) of four healthy Beagle dogs. Femur served as non-oral control. Clonogenic and proliferative abilities were assessed. In vitro osteogenic, chondrogenic, adipogenic and neural multilineage differentiation were correlated with in vivo bone regeneration and potential for clinical applications. M-cMSCs displayed two-fold increase in clonogenic and proliferative capacities relative to F-cMSCs (p =0.006). M-cMSCs in vitro osteogenesis based on alkaline phosphatase (p =0.04), bone sialoprotein (p =0.05), and osteocalcin (p =0.03), as well as adipogenesis (p =0.007), and chondrogenesis (p =0.009) were relatively higher and correlated with enhanced M-cMSC bone regenerative capacity. Neural expression markers, nestin and βIII-tubulin were not significantly different. The enhanced differentiation and bone regenerative capacity of mandible MSCs may make them favorable donor graft materials for site-specific jaw bone regeneration.
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影响因子：
7.5
作者：
Akiyama K;You YO;Yamaza T;Chen C;Tang L;Jin Y;Chen XD;Gronthos S;Shi S
通讯作者：
Shi S
DOI：
10.1016/j.oooo.2013.05.023
发表时间：
2013-09
期刊：
Oral surgery, oral medicine, oral pathology and oral radiology
影响因子：
--
作者：
Akintoye SO;Boyce AM;Collins MT
通讯作者：
Collins MT
影响因子：
4.2
作者：
Dissanayaka, Waruna Lakmal;Zhu, Xiaofei;Jin, Lijian
通讯作者：
Jin, Lijian
影响因子：
2.5
作者：
Kim, Yongsoo;Leem, Dae Ho
通讯作者：
Leem, Dae Ho
DOI：
10.1016/j.tripleo.2011.05.032
发表时间：
2011-12-01
期刊：
ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY AND ENDODONTOLOGY
影响因子：
--
作者：
Lee, Bu-Kyu;Choi, Su-Jin;Oh, Se-Hoon
通讯作者：
Oh, Se-Hoon