Whole-Transcriptome Analysis of LncRNAs Mediated ceRNA Regulation in Granulosa Cells Isolated From Healthy and Atresia Follicles of Chinese Buffalo.
Whole-Transcriptome Analysis of LncRNAs Mediated ceRNA Regulation in Granulosa Cells Isolated From Healthy and Atresia Follicles of Chinese Buffalo.
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从中国水牛健康和闭锁卵泡中分离的颗粒细胞中 LncRNA 介导的 ceRNA 调节的全转录组分析
DOI：
10.3389/fvets.2021.680182
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发表时间：
2021
影响因子：
3.2
通讯作者：
Deng Y
中科院分区：
文献类型：
--
作者：
Pan Y;Yang S;Cheng J;Lv Q;Xing Q;Zhang R;Liang J;Shi D;Deng Y
颗粒细胞是卵泡的主要支持细胞，在卵母细胞成熟和卵泡闭锁的调控中起重要作用。越来越多的证据表明非编码RNA参与了GC生理功能的调节。然而，布法罗GC的全转录组分析尚未报道。本研究根据卵泡液中GC凋亡率和激素水平将健康卵泡（HF）和闭锁卵泡（AF）区分开来。从HF和AF（n = 15，5 < n < 8 mm）收集GC，用于使用第二代高通量测序的全转录组分析。共1，861和1，075个mRNA，159和24个miRNA，123和100个lncRNA分别在HF和AF之间上调和下调。这些差异表达基因的功能和信号通路的丰富表明，大多数DEmRNAs和DEmiRNAs的靶点被注释到ECM-受体相互作用和粘着斑的类别，以及PI 3 K-AKT，mTOR，TGF-β，Rap 1和雌激素信号通路。基于竞争性内源RNA（CeRNA）理论构建了竞争性内源RNA网络，进一步揭示了这些DERNA在布法罗卵泡GC中的调控作用。最后，我们验证了lnc 4040通过ceRNA机制调控Hif 1a作为miR-709海绵体的表达，提示其在布法罗卵泡GC中的重要功能。这些结果表明lncRNA在HFs和AFs的GC中动态表达，并以ceRNA方式与靶基因相互作用，提示其在布法罗卵泡发育和闭锁中的重要作用。
Granulosa cells (GCs) are the main supporting cells in follicles and play an important role in the regulation of oocyte maturation and follicular atresia. Accumulating evidence indicates that non-coding RNAs participate in regulation of the physiological function of GCs. However, whole-transcriptome analysis for GCs of buffalo has yet to be reported. In this study, healthy follicles (HFs) and atretic follicles (AFs) were defined according to the apoptosis rate of GCs and the hormone level in follicular fluid. GCs were collected from HFs and AFs (n = 15, 5 < n < 8 mm) for whole-transcriptome analysis using second-generation high-throughput sequencing. A total of 1,861 and 1,075 mRNAs, 159 and 24 miRNAs, and 123 and 100 lncRNAs, were upregulated and downregulated between HFs and AFs, respectively. Enrichment of functions and signaling pathways of these differentially expressed (DE) genes showed that most of DEmRNAs and targets of DEmiRNAs were annotated to the categories of ECM–receptor interaction and focal adhesion, as well as PI3K-AKT, mTOR, TGF-beta, Rap1, and estrogen signaling pathways. The competing endogenous RNA (CeRNA) network was also constructed based on the ceRNA theory which further revealed regulatory roles of these DERNAs in GCs of buffalo follicles. Finally, we validated that lnc4040 regulated the expression of Hif1a as miR-709 sponge in a ceRNA mechanism, suggesting their critical functions in GCs of buffalo follicles. These results show that lncRNAs are dynamically expressed in GCs of HFs and AFs, and interacting with target genes in a ceRNA manner, suggesting their critical functions in buffalo follicular development and atresia.
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DOI：
10.1002/jcsm.12266
发表时间：
2018-04
期刊：
Journal of cachexia, sarcopenia and muscle
影响因子：
--
作者：
Connolly M;Paul R;Farre-Garros R;Natanek SA;Bloch S;Lee J;Lorenzo JP;Patel H;Cooper C;Sayer AA;Wort SJ;Griffiths M;Polkey MI;Kemp PR
通讯作者：
Kemp PR
影响因子：
4
作者：
Feng, Xu;Li, Fengzhe;Zhang, Yanli
通讯作者：
Zhang, Yanli
影响因子：
3.6
作者：
Fiedler, Stephanie D.;Carletti, Martha Z.;Christenson, Lane K.
通讯作者：
Christenson, Lane K.
影响因子：
2.8
作者：
Feng, W.-G.;Sui, H.-S.;Tan, J.-H.
通讯作者：
Tan, J.-H.
PBX3 是多种 miRNA 的靶标，对于肝脏肿瘤起始细胞至关重要
影响因子：
16.6
作者：
Han, Haibo;Du, Yantao;Zhang, Zhiqian
通讯作者：
Zhang, Zhiqian