Activating and repressing gene expression between chromosomes during stochastic fate specification.
Activating and repressing gene expression between chromosomes during stochastic fate specification.
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DOI：
10.1016/j.celrep.2022.111910
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发表时间：
2023-01-31
期刊：
影响因子：
8.8
通讯作者：
中科院分区：
文献类型：
--
作者：
DNA元件跨基因组距离作用，调控基因表达。在果蝇的横切过程中，一个基因的一个等位基因上的DNA元件在染色体之间起作用，调节另一个等位基因的表达。对转染的生物学作用和发育调控知之甚少。在此，我们研究了蝇眼中无棘（ss）光感受器的随机表达，以了解横切。我们确定了一个生物学作用，横切在调节自然发生的ss等位基因表达。我们鉴定了激活和抑制横切所需的DNA元件。不同的增强子在发育的不同时期参与转染，促进基因表达和指定细胞命运。将异源染色体上的沉默元件与ss位点相邻“重建”该基因，导致抑制。我们的研究表明，横切在发育的特定时间点通过不同的DNA元件调节基因表达，对基因组的组织和结构有影响。R7光感受器亚型在蝇眼中以2:1的比例随机分布。横断介导决定命运的表达。Urban等人发现，无脊椎病的野生等位基因之间发生了横切。他们剖析了这一过程所需的激活和抑制DNA元素。
DNA elements act across long genomic distances to regulate gene expression. During transvection in Drosophila, DNA elements on one allele of a gene act between chromosomes to regulate expression of the other allele. Little is known about the biological roles and developmental regulation of transvection. Here, we study the stochastic expression of spineless (ss) in photoreceptors in the fly eye to understand transvection. We determine a biological role for transvection in regulating expression of naturally occurring ss alleles. We identify DNA elements required for activating and repressing transvection. Different enhancers participate in transvection at different times during development to promote gene expression and specify cell fates. Bringing a silencer element on a heterologous chromosome into proximity with the ss locus “reconstitutes” the gene, leading to repression. Our studies show that transvection regulates gene expression via distinct DNA elements at specific timepoints in development, with implications for genome organization and architecture. R7 photoreceptor subtypes are randomly patterned in the fly eye in a 2:1 ratio. Transvection mediates expression of the fate determinant spineless. Urban et al. find that transvection occurs between wild-derived alleles of spineless. They dissect the activating and repressive DNA elements required for this process.
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DOI：
10.1083/jcb.141.1.5
发表时间：
1998-04-06
期刊：
The Journal of cell biology
影响因子：
--
作者：
Fung JC;Marshall WF;Dernburg A;Agard DA;Sedat JW
通讯作者：
Sedat JW
影响因子：
64.5
作者：
Czermin, B;Melfi, R;Pirrotta, V
通讯作者：
Pirrotta, V
影响因子：
10.5
作者：
FAUVARQUE, MO;DURA, JM
通讯作者：
DURA, JM
影响因子：
10.5
作者：
Duncan, DM;Burgess, EA;Duncan, I
通讯作者：
Duncan, I
影响因子：
7.7
作者：
Anderson, Caitlin;Reiss, India;Johnston, Robert J.
通讯作者：
Johnston, Robert J.