Temporal Coordination of Carbohydrate Metabolism during Mosquito Reproduction.
Temporal Coordination of Carbohydrate Metabolism during Mosquito Reproduction.
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蚊子繁殖过程中碳水化合物代谢的时间协调
DOI：
10.1371/journal.pgen.1005309
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发表时间：
2015-07
期刊：
影响因子：
4.5
通讯作者：
Zou Z
中科院分区：
文献类型：
--
作者：
Hou Y;Wang XL;Saha TT;Roy S;Zhao B;Raikhel AS;Zou Z
食血蚊子是多种毁灭性人类疾病的媒介，许多独特的生理特征促成了这些昆虫令人难以置信的进化成功。这些功能对繁殖的雌性蚊子提出了高能量需求，碳水化合物代谢(CM)必须与这些需求同步。代谢基因谱的功能分析表明，主要的CM途径，包括糖酵解、糖原和糖代谢以及柠檬酸循环，在蚊子脂肪体内羽化后(PE)阶段受到显著抑制，在血餐后(PBM)阶段急剧增加，Real-Time RT-PCR也证实了这一点。与CM基因转录水平和蛋白水平的变化相一致，糖原、葡萄糖和海藻糖等次生代谢物水平在生殖周期中也分别周期性地积累和降解。三酰甘油(TAG)是蚊子脂肪体内另一种重要的能量储存形式，其水平也遵循类似的趋势。另一方面，由这些次生代谢物分解产生的ATP则显示出相反的趋势。此外，我们使用了对保幼激素和蜕皮激素受体Met和ECR的RNA干扰研究，结合转录和代谢组学分析表明，这些激素受体作为主要的调节开关，协调CM与雌性蚊子在其生殖周期中不同的能量需求。我们的研究表明，通过代谢重新编程，多细胞有机体如何适应剧烈和快速的功能变化。蚊子传播许多毁灭性的人类疾病，因为它们必须吞噬血液，这是有效繁殖所必需的。新陈代谢必须与雌性蚊子寻找宿主、吸血和快速卵子发育的高能量需求同步。每个生殖周期分为两个阶段，依次由保幼激素(JH)和20-羟基蜕皮激素(20-羟基蜕皮激素)控制。在血餐前阶段，JH受体甲基戊二烯耐受(Met)控制着碳水化合物代谢(CM)途径，其RNA干扰(RNAi)沉默导致CM酶在转录和蛋白质水平上的上调，激活糖酵解通量，并耗尽储存和循环糖。在第二阶段，血餐后，CM受到蜕皮激素受体ECR的调节，其RNAi沉默的作用与Met RNAi相反。因此，我们发现Met和ECR是调节雌性蚊子生殖周期中碳水化合物代谢和能量需求的调节开关。
Hematophagous mosquitoes serve as vectors of multiple devastating human diseases, and many unique physiological features contribute to the incredible evolutionary success of these insects. These functions place high-energy demands on a reproducing female mosquito, and carbohydrate metabolism (CM) must be synchronized with these needs. Functional analysis of metabolic gene profiling showed that major CM pathways, including glycolysis, glycogen and sugar metabolism, and citrate cycle, are dramatically repressed at post eclosion (PE) stage in mosquito fat body followed by a sharply increase at post-blood meal (PBM) stage, which were also verified by Real-time RT-PCR. Consistent to the change of transcript and protein level of CM genes, the level of glycogen, glucose and trehalose and other secondary metabolites are also periodically accumulated and degraded during the reproductive cycle respectively. Levels of triacylglycerols (TAG), which represent another important energy storage form in the mosquito fat body, followed a similar tendency. On the other hand, ATP, which is generated by catabolism of these secondary metabolites, showed an opposite trend. Additionally, we used RNA interference studies for the juvenile hormone and ecdysone receptors, Met and EcR, coupled with transcriptomics and metabolomics analyses to show that these hormone receptors function as major regulatory switches coordinating CM with the differing energy requirements of the female mosquito throughout its reproductive cycle. Our study demonstrates how, by metabolic reprogramming, a multicellular organism adapts to drastic and rapid functional changes. Mosquitoes transmit numerous devastating human diseases due to their obligatory hematophagy that is required for the efficient reproduction. Metabolism must be synchronized with high energetic needs of a female mosquito for host seeking, blood feeding and rapid egg development. Each reproductive cycle is divided into two phases that are sequentially governed by juvenile hormone (JH) and 20-hydroxyecdysone. During the pre-blood meal phase, the JH receptor Methoprene-tolerant (Met) controls carbohydrate metabolism (CM) pathways and its RNA interference (RNAi) silencing caused up-regulation of CM enzymes at the transcript and protein levels activating glycolytic flux and depletion of storage and circulating sugars. During the second, post blood meal phase, CM was regulated by the ecdysone receptor EcR and its RNAi silencing had a dramatic effect opposite to that of Met RNAi. Thus, we show that Met and EcR function as regulatory switches coordinating carbohydrate metabolism with energetic requirements of the female mosquito reproductive cycle.
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影响因子：
13.8
作者：
Patra, Krushna C.;Hay, Nissim
通讯作者：
Hay, Nissim
DOI：
10.1073/pnas.1116123109
发表时间：
2011-12-27
影响因子：
11.1
作者：
Charles, Jean-Philippe;Iwema, Thomas;Jindra, Marek
通讯作者：
Jindra, Marek
影响因子：
2.5
作者：
Martins, Gustavo Ferreira;Serrao, Jose Eduardo;Ramalho-Ortigao, Jose Marcelo;Paolucci Pimenta, Paulo Filemon
通讯作者：
Paolucci Pimenta, Paulo Filemon
影响因子：
3.8
作者：
Raikhel, AS;Kokoza, VA;Attardo, G
通讯作者：
Attardo, G
影响因子：
3.7
作者：
Bryant B;Raikhel AS
通讯作者：
Raikhel AS