Histochemical and ultrastructural studies of the mosquito Aedes aegypti fat body: effects of aging and diet type.
Histochemical and ultrastructural studies of the mosquito Aedes aegypti fat body: effects of aging and diet type.
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DOI：
10.1002/jemt.20990
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发表时间：
2011-11
影响因子：
2.5
通讯作者：
Paolucci Pimenta, Paulo Filemon
中科院分区：
文献类型：
--
作者：
Martins, Gustavo Ferreira;Serrao, Jose Eduardo;Ramalho-Ortigao, Jose Marcelo;Paolucci Pimenta, Paulo Filemon
埃及伊蚊是世界范围内登革热的主要媒介，也是城市黄热病的主要媒介。尽管它在流行病学上很重要，但人们对这种蚊子脂肪体的细胞和结构变化知之甚少。在这里，我们应用光镜和透射电镜来研究三组A.埃及雌性：新出现的，18日龄的糖喂养的，和18日龄的血喂养的。脂肪体由一层附着在腹部珠被上的细胞组成，由滋养细胞和卵母细胞形成。滋养细胞对碳水化合物呈强阳性，而卵母细胞对蛋白质和脂质呈强阳性。超微结构分析表明，这些细胞富含糖原和游离核糖体。还检测到在血餐后分解的许多脂滴和蛋白质颗粒。在18日龄的糖饲料中，滋养细胞显示出充满脂滴的混乱的细胞质，游离核糖体、糖原、粗面内质网（RER）和线粒体的数量减少。在血餐之后，粗面内质网和线粒体显示出增大的尺寸，表明活性增加。在卵母细胞方面，这些细胞显示出电子致密的细胞质和面向血淋巴的质膜内折。作为A.埃及雌蚊摄食血液后，滋养细胞和卵母细胞核增大，但直径减小。我们的研究结果表明，滋养细胞和卵母细胞的重塑可能参与了脂肪体的功能变化，发生在衰老过程中，并在A。埃及女性。
Aedes aegypti is the principal vector of dengue world wide and a major vector of urban yellow fever. Despite its epidemiological importance, not much is known regarding cellular and structural changes in the fat body in this mosquito. Here, we applied light and transmission electron microscopies in order to investigate structural changes in the fat body of three groups of A. aegypti females: newly emerged, 18-day-old sugar-fed, and 18-day-old blood-fed. The fat body consists of a layer of cells attached to the abdomen integument, formed by trophocytes and oenocytes. Trophocytes are strongly positive for carbohydrates, while oenocytes are strongly positive for proteins and lipids. Ultrastructural analyses of trophocytes from newly emerged and 18-day-old blood-fed indicate that these cells are rich in glycogen and free ribosomes. Many lipid droplets and protein granules, which are broken down after the blood meal, are also detected. In 18-day-old sugar-fed, trophocytes display a disorganized cytoplasm filled with lipid droplets, and reduced numbers of free ribosomes, glycogen, rough endoplasmic reticulum (RER) and mitochondria. Following a blood meal, the RER and mitochondria display enlarged sizes, suggestive of increased activity. In regards to oenocytes, these cells display an electron-dense cytoplasm and plasma membrane infoldings facing the hemolymph. As the A. aegypti female ages, trophocyte and oenocyte cell nuclei become larger but decrease in diameter after blood feeding. Our findings suggest that the trophocytes and oenocytes remodeling is likely involved in functional changes of fat body that take place during aging and following a blood meal in A. aegypti females.
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影响因子：
2.1
作者：
PIMENTA, PFP;DESOUZA, W
通讯作者：
DESOUZA, W
影响因子：
3.6
作者：
STOPPIE, P;BRIERS, T;DELOOF, A
通讯作者：
DELOOF, A
影响因子：
--
作者：
BUTTERWORTH, FM;RASCH, EM
通讯作者：
RASCH, EM
影响因子：
2.2
作者：
GRZELAK, K;KUMARAN, AK
通讯作者：
KUMARAN, AK
影响因子：
2.2
作者：
RAIKHEL, AS
通讯作者：
RAIKHEL, AS