A blend of small molecules regulates both mating and development in Caenorhabditis elegans.
A blend of small molecules regulates both mating and development in Caenorhabditis elegans.
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DOI：
10.1038/nature07168
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发表时间：
2008-08-28
期刊：
影响因子：
64.8
通讯作者：
Schroeder, Frank C.
中科院分区：
文献类型：
--
作者：
Srinivasan, Jagan;Kaplan, Fatma;Ajredini, Ramadan;Zachariah, Cherian;Alborn, Hans T.;Teal, Peter E. A.;Malik, Rabia U.;Edison, Arthur S.;Sternberg, Paul W.;Schroeder, Frank C.
在许多生物中，种群密度感应和性吸引依赖于小分子信号系统。在线虫秀丽隐杆线虫中，通过双脱氧糖蛔虫糖的特异性糖苷（“蛔虫糖”）监测种群密度，该糖苷促进进入另一个幼虫阶段，即非进食和高度持久的dauer阶段。此外，成年C.雄性秀丽线虫被一种以前未被发现的小分子信号吸引到雌雄同体。在这里，我们表明，通过组合活性导向分馏的C。elegans代谢组，交配信号由三种dauer诱导的ascarosides的协同混合物组成，我们称之为ascr#2，ascr#3和ascr#4。这种混合物的蛔虫苷作为一个有效的男性引诱剂在非常低的浓度，而在较高的浓度所需的dauer形成的化合物不再吸引男性，而是阻止雌雄同体。Ascarosides ascr#2和ascr#3携带不同但重叠的信息，因为ascr#3作为雄性引诱剂比ascr#2更有效，而ascr#2在促进dauer形成方面比ascr#3稍微更有效。我们证明，ascr#2，ascr#3和ascr#4是强烈的协同作用，两种类型的神经元，两栖类单纤毛感觉神经元类型K（ASK）和男性特定的头伴侣神经元（CEM），所需的男性吸引力ascr#3。在此基础上，对中国竹荪的雄性吸引和雌雌虫的形成进行了初步的研究。线虫似乎是对一组共同的信号分子的替代行为反应。因此，蛔虫苷信号系统连接生殖和发育途径，并代表了信号分子的结构和浓度依赖性差异活性的一个独特的例子。
In many organisms, population-density sensing and sexual attraction rely on small-molecule-based signalling systems. In the nematode Caenorhabditis elegans, population density is monitored through specific glycosides of the dideoxysugar ascarylose (the `ascarosides') that promote entry into an alternative larval stage, the non-feeding and highly persistent dauer stage. In addition, adult C. elegans males are attracted to hermaphrodites by a previously unidentified small-molecule signal. Here we show, by means of combinatorial activity-guided fractionation of the C. elegans metabolome, that the mating signal consists of a synergistic blend of three dauer-inducing ascarosides, which we call ascr#2, ascr#3 and ascr#4. This blend of ascarosides acts as a potent male attractant at very low concentrations, whereas at the higher concentrations required for dauer formation the compounds no longer attract males and instead deter hermaphrodites. The ascarosides ascr#2 and ascr#3 carry different, but overlapping, information, as ascr#3 is more potent as a male attractant than ascr#2, whereas ascr#2 is slightly more potent than ascr#3 in promoting dauer formation. We demonstrate that ascr#2, ascr#3 and ascr#4 are strongly synergistic, and that two types of neuron, the amphid single-ciliated sensory neuron type K (ASK) and the male-specific cephalic companion neuron (CEM), are required for male attraction by ascr#3. On the basis of these results, male attraction and dauer formation in C. elegans appear as alternative behavioural responses to a common set of signalling molecules. The ascaroside signalling system thus connects reproductive and developmental pathways and represents a unique example of structure- and concentration-dependent differential activity of signalling molecules.
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影响因子：
4.6
作者：
Bae, Young-Kyung;Qin, Hongmin;Barr, Maureen M.
通讯作者：
Barr, Maureen M.
影响因子：
2.7
作者：
HIRSH, D;OPPENHEIM, D;KLASS, M
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影响因子：
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作者：
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DOI：
10.1073/pnas.032225799
发表时间：
2002-02-05
影响因子：
11.1
作者：
Simon, JM;Sternberg, PW
通讯作者：
Sternberg, PW
影响因子：
2.9
作者：
Cronin CJ;Mendel JE;Mukhtar S;Kim YM;Stirbl RC;Bruck J;Sternberg PW
通讯作者：
Sternberg PW