Oxidative stress is involved in the pathogenesis of Keshan disease (an endemic dilated cardiomyopathy) in China.
Oxidative stress is involved in the pathogenesis of Keshan disease (an endemic dilated cardiomyopathy) in China.
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氧化应激与中国克山病（一种地方性扩张型心肌病）的发病机制有关。
DOI：
10.1155/2013/474203
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发表时间：
2013
影响因子：
--
通讯作者：
Liu Y
中科院分区：
文献类型：
--
作者：
Pei J;Fu W;Yang L;Zhang Z;Liu Y
氧化应激和硒蛋白缺乏被认为与克山病（KD）的发病机制有关。然而，据我们所知，尚未对川崎病患者心肌中的氧化应激水平和硒蛋白表达进行研究。本研究采用氧化应激标志物8-羟基-2-脱氧鸟苷（8-OH-dG）来评估氧化应激水平，并评估硫氧还蛋白还原酶1（TrxR1）和谷胱甘肽过氧化物酶1（GPx1）来反映硒蛋白水平。对 8 名 KD 患者和 9 名非 KD 患者（对照）的心肌样本进行了 8-OH-dG、TrxR1 和 GPx1 的免疫组织化学染色。随后使用 Olympus Image-Pro Plus 6.0 软件对染色强度进行量化。数据显示，KD组心肌细胞核8-OH-dG表达阳性率（68.6%）高于对照组（2.4%）。此外，KD组8-OH-dG阳性率与心肌损伤程度呈正相关。 TrxR1和GPx-1的分布与心肌损伤的分布无关。这两种硒蛋白的表达在对照组中高于KD组。我们的研究是关于川崎病患者心肌中氧化应激和硒蛋白表达谱的第一份报告。 KD患者氧化应激水平显着升高且与心肌损伤程度呈正相关。硒蛋白 TrxR1 和 GPx1 可能在 KD 的发病机制中发挥作用。
Oxidative stress and selenoprotein deficiency are thought to be associated with the pathogenesis of Keshan disease (KD). However, to our knowledge, the level of oxidative stress and expression of selenoproteins have not been investigated in the myocardium of patients with KD. In this study, 8-hydroxy-2-deoxy guanosine (8-OH-dG), a marker of oxidative stress, was used to assess the level of oxidative stress, and thioredoxin reductase 1 (TrxR1) and glutathione peroxidase 1 (GPx1) were assessed to reflect the level of selenoproteins. Myocardial samples from 8 patients with KD and 9 non-KD patients (controls) were immunohistochemically stained for 8-OH-dG, TrxR1, and GPx1. The staining intensities were subsequently quantified using Olympus Image-Pro Plus 6.0 software. The data showed that the positive rate of 8-OH-dG expression in myocardial nuclei was higher in the KD group (68.6%) than that in the control group (2.4%). In addition, a positive correlation between the positive rate of 8-OH-dG and the degree of myocardial damage was observed in the KD group. The distribution of TrxR1 and GPx-1 was not associated with the distribution of myocardial damage. The expression of these two selenoproteins was higher in the control group than that in the KD group. Our study represents the first report on the expression profiles of oxidative stress and selenoproteins in the myocardium of patients with KD. The level of oxidative stress significantly increased and was positively correlated with the degree of myocardial damage in patients with KD. The selenoproteins, TrxR1 and GPx1, may have a role in the pathogenesis of KD.
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影响因子：
4.2
作者：
Lei, Cong;Niu, Xiaolin;Wei, Jin
通讯作者：
Wei, Jin
影响因子：
4.1
作者：
Khalil, W. K. B.;Girgis, E.;Rao, K. V.
通讯作者：
Rao, K. V.
影响因子：
158.5
作者：
Blankenberg, S;Rupprecht, HJ;Lackner, KJ
通讯作者：
Lackner, KJ
影响因子：
20.1
作者：
Cesselli, D;Jakoniuk, I;Anversa, P
通讯作者：
Anversa, P
影响因子：
4.8
作者：
Lubos, Edith;Kelly, Neil J.;Handy, Diane E.
通讯作者：
Handy, Diane E.