Genipin crosslinking decreases the mechanical wear and biochemical degradation of impacted cartilage in vitro.
Genipin crosslinking decreases the mechanical wear and biochemical degradation of impacted cartilage in vitro.
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DOI：
10.1002/jor.23411
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发表时间：
2017-03
期刊：
影响因子：
--
通讯作者：
Wagner DR
中科院分区：
文献类型：
--
作者：
Bonitsky CM;McGann ME;Selep MJ;Ovaert TC;Trippel SB;Wagner DR
软骨的高能量创伤会导致表面裂缝和微结构损伤，但这种损伤使组织更容易磨损并导致创伤后骨关节炎（PTOA）进展的程度尚不清楚。此外，目前没有治疗方法可用于在关节创伤后强化软骨并保护组织免受随后的降解和磨损。本研究的目的是调查的作用，机械损伤的降解和磨损的软骨，以评估影响和随后的京尼平交联对关节软骨的粘弹性参数的变化的影响，并测试的假设，京尼平交联是一种有效的治疗，以提高抵抗生化降解和机械磨损。结果表明，软骨刚度降低后，冲击负荷，可能是由于裂缝和微结构损伤的形成，并通过交联部分或全部恢复。与未受损软骨相比，受影响的关节软骨的耐磨性降低，这表明由冲击直接引起的机械损伤可能有助于PTOA的进展。然而，耐磨性的降低被交联处理完全逆转。此外，交联处理改善了撞击损伤关节面对胶原酶消化的抵抗力。这些结果突出了通过京尼平交联胶原蛋白在预防创伤性损伤后软骨变性中的潜在治疗价值。
High energy trauma to cartilage causes surface fissures and microstructural damage, but the degree to which this damage renders the tissue more susceptible to wear and contributes to the progression of post-traumatic osteoarthritis (PTOA) is unknown. Additionally, no treatments are currently available to strengthen cartilage after joint trauma and to protect the tissue from subsequent degradation and wear. The purposes of this study were to investigate the role of mechanical damage in the degradation and wear of cartilage, to evaluate the effects of impact and subsequent genipin crosslinking on the changes in the viscoelastic parameters of articular cartilage, and to test the hypothesis that genipin crosslinking is an effective treatment to enhance the resistance to biochemical degradation and mechanical wear. Results demonstrate that cartilage stiffness decreases after impact loading, likely due to the formation of fissures and microarchitectural damage, and is partially or fully restored by crosslinking. The wear resistance of impacted articular cartilage was diminished compared to undamaged cartilage, suggesting that mechanical damage that is directly induced by the impact may contribute to the progression of PTOA. However, the decrease in wear resistance was completely reversed by the crosslinking treatments. Additionally, the crosslinking treatments improved the resistance to collagenase digestion at the impact-damaged articular surface. These results highlight the potential therapeutic value of collagen crosslinking via genipin in the prevention of cartilage degeneration after traumatic injury.
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