Distribution and nature of epoxide hydrolase activity in subcellular organelles of mouse liver.
Distribution and nature of epoxide hydrolase activity in subcellular organelles of mouse liver.
复制标题
小鼠肝脏亚细胞器中环氧化物水解酶活性的分布和性质。
DOI：
10.1016/0006-2952(86)90275-3
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发表时间：
1986
影响因子：
5.8
通讯作者：
Gill,SS
中科院分区：
文献类型：
--
作者：
Kaur,S;Gill,SS
Mouse liver light and heavy mitochondrial fractions contain significant epoxide hydrolase activity in addition to that present in the cytosol and microsomes. As the mitochondrial fraction itself contains a number of subfractions, experiments were designed to determine the localization of the epoxide hydrolase activity in these subfractions. Subcellular fractions were prepared using livers from 6- to 8-week-old Swiss-Webster male mice. Usingtrans-stilbene oxide (TSO) as substrate, the highest activity was localized in the cytosolic fraction, followed by the light mitochondrial fraction. Subfractionation of the light mitochondrial fraction by isopycnic sucrose density gradient resulted in the separation of mitochondria from peroxisomes as monitored by marker enzymes. The separation of these two subcellular organelles was also confirmed by the electron microscopic studies. Distribution of TSO-hydrolase activity in the sucrose density gradient fractions closely resembled the activity distribution of the peroxisomal markers catalase and urate oxidase, but significant activity was also found in mitochondria. Treatment of mice with clofibrate selectively induced TSO-hydrolase in the cytosol without affecting this enzyme activity in the peroxisomal fraction. There was no difference in the distribution pattern of TSO-hydrolase and marker enzymes in sucrose density gradients of mitochondrial fractions from clofibrate-treated and control mice. The epoxide hydrolase activity in the peroxisomes is immunologically similar to, and also has the same molecular weight as, the cytosolic epoxide hydrolase.
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小鼠肝脏线粒体部分中的环氧化物水解酶活性
影响因子：
64.8
作者：
S. Gill;B. Hammock
通讯作者：
B. Hammock
大鼠肝核膜的环氧化物水酶和混合功能氧化酶活性。
影响因子：
3.9
作者：
H. Mukhtar;T. H. Elmamlouk;J. Bend
通讯作者：
J. Bend
使用垂直转子从大鼠肝脏快速制备过氧化物酶体。
DOI：
10.1042/bj1800445
发表时间：
1979
期刊：
The Biochemical journal
影响因子：
--
作者：
C. Neat;H. Osmundsen
通讯作者：
H. Osmundsen
大鼠和小鼠中的胞质环氧化物水解酶和微粒体环氧化物水解酶在免疫学上是可区分的。
DOI：
10.1016/s0021-9258(19)69582-7
发表时间：
1981
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
T. Guenthner;B. Hammock;U. Vogel;F. Oesch
通讯作者：
F. Oesch
影响因子：
4.8
作者：
M. Swanson
通讯作者：
M. Swanson