Expression of hypoxia-inducible factor 1 alpha and oligodendrocyte lineage gene-1 in cultured brain slices after oxygen-glucose deprivation.
Expression of hypoxia-inducible factor 1 alpha and oligodendrocyte lineage gene-1 in cultured brain slices after oxygen-glucose deprivation.
复制标题
氧糖剥夺后培养脑切片中缺氧诱导因子 1 α 和少突胶质细胞谱系基因 1 的表达
DOI：
10.3969/j.issn.1673-5374.2013.04.005
复制
发表时间：
2013-02-05
影响因子：
6.1
通讯作者：
Chang Y
中科院分区：
文献类型：
--
作者：
Cui H;Han W;Yang L;Chang Y
少突胶质细胞谱系基因-1在少突胶质细胞中表达，可能引发神经髓鞘损伤的修复，并在髓鞘修复中发挥关键作用。缺氧诱导因子1α是一种转录因子，在早产儿缺氧缺血性脑损伤中具有重要意义。缺氧诱导因子1α对少突胶质细胞谱系基因-1的直接调控作用尚缺乏证据。本研究采用Sprague-Dawley大鼠脑切片培养，进行氧-葡萄糖剥夺。然后，用缺氧诱导因子1α或少突胶质细胞谱系基因-1转染切片。免疫组化染色检测转染前大鼠脑缺氧诱导因子1α和少突胶质细胞谱系基因1的表达水平显著上调。缺氧诱导因子1α转染8 h后，少突胶质细胞谱系基因-1表达上调，并在转染24 h后达到峰值。少突胶质细胞谱系基因-1转染对缺氧-葡萄糖剥夺大鼠脑组织缺氧诱导因子1α水平无显著影响。提示缺氧诱导因子1α可调节缺氧脑组织少突胶质细胞谱系基因-1的表达，从而修复神经损伤。
Oligodendrocyte lineage gene-1 expressed in oligodendrocytes may trigger the repair of neuronal myelin impairment, and play a crucial role in myelin repair. Hypoxia-inducible factor 1α, a transcription factor, is of great significance in premature infants with hypoxic-ischemic brain damage. There is little evidence of direct regulatory effects of hypoxia-inducible factor 1α on oligodendrocyte lineage gene-1. In this study, brain slices of Sprague-Dawley rats were cultured and subjected to oxygen-glucose deprivation. Then, slices were transfected with hypoxia-inducible factor 1α or oligodendrocyte lineage gene-1. The expression levels of hypoxia-inducible factor 1α and oligodendrocyte lineage gene-1 were significantly up-regulated in rat brains prior to transfection, as detected by immunohistochemical staining. Eight hours after transfection of slices with hypoxia-inducible factor 1α, oligodendrocyte lineage gene-1 expression was upregulated, and reached a peak 24 hours after transfection. Oligodendrocyte lineage gene-1 transfection induced no significant differences in hypoxia-inducible factor 1α levels in rat brain tissues with oxygen-glucose deprivation. These experimental findings indicate that hypoxia-inducible factor 1α can regulate oligodendrocyte lineage gene-1 expression in hypoxic brain tissue, thus repairing the neural impairment.
登录
查看更多内容
影响因子：
5.3
作者：
Keir, SD;House, SB;Gainer, H
通讯作者：
Gainer, H
影响因子：
2.9
作者：
Guo, Rong;Hou, Weijian;Weiner, Carl P.
通讯作者：
Weiner, Carl P.
影响因子：
3.2
作者：
Aboul-Enein, F;Rauschka, H;Lassmann, H
通讯作者：
Lassmann, H
影响因子：
4.7
作者：
Lee, Yoon-Mi;Lim, Ji-Hong;Park, Jong-Wan
通讯作者：
Park, Jong-Wan
影响因子：
11.2
作者：
Kinney, Hannah C.;Haynes, Robin L.;Xu, Gang;Andiman, Sarah E.;Folkerth, Rebecca D.;Sleeper, Lynn A.;Volpe, Joseph J.
通讯作者：
Volpe, Joseph J.