Development of marker-free rice with stable and high resistance to rice black-streaked dwarf virus disease through RNA interference.
Development of marker-free rice with stable and high resistance to rice black-streaked dwarf virus disease through RNA interference.
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利用RNA干扰技术培育稳定高抗水稻黑条矮缩病的无标记水稻。
DOI：
10.1111/pbi.13459
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发表时间：
2021-03
影响因子：
13.8
通讯作者：
Zuo S
中科院分区：
文献类型：
--
作者：
Feng Z;Yuan M;Zou J;Wu LB;Wei L;Chen T;Zhou N;Xue W;Zhang Y;Chen Z;Hu K;Wang GL;Liu W;Pan X;Zuo S
水稻黑条矮缩病毒（RBSDV）病在亚洲造成严重的水稻产量损失。RNA干扰技术已广泛应用于植物抗病品种的培育。迄今为止，RNAi在水稻抗RBSDV方面的应用研究较少，且大多缺乏足够的数据支持其育种潜力，限制了抗RBSDV品种的开发进展。在本研究中，我们构建了三个RNAi构建体，分别特异性靶向三个RBSDV基因（S1， S2和S6）。通过连续8年的人工接种和田间试验的表型检测，我们证实了RNAi靶向RBSDV S6使水稻对RBSDV具有几乎完全的免疫力，而靶向S1或S2的RNAi只导致部分抗性增强。S6RNAi还被发现对南方水稻黑条矮缩病毒（SRBSDV）具有很强的抗性，SRBSDV是最近在中国南方爆发的一种与RBSDV密切相关的新物种。在S6RNAi转基因品系中，未发现对农艺性状和发育性状产生不良影响。由玉米泛素- 1启动子或水稻红细胞绿色组织表达启动子驱动的无标记S6RNAi转基因品系，在水稻优良背景下，在培育抗RBSDV和SRBSDV的品种方面具有很大的潜力，并为进一步的安全性评价和商业应用提供了基础。
The rice black‐streaked dwarf virus (RBSDV) disease causes severe rice yield losses in Asia. RNA interference (RNAi) has been widely applied to develop antiviral varieties in plants. So far, only a few studies reported the application of RNAi in rice against RBSDV and most of them are lack of enough data to support its breeding potential, which limited the progress on developing RBSDV‐resistant variety. In this study, we generated three RNAi constructs to specifically target three RBSDV genes (S1, S2 and S6), respectively. We confirmed that RNAi targeting RBSDV S6 conferred rice with almost full immunity to RBSDV through phenotyping test in eight consecutive years in both artificial inoculation and field trials, while RNAi of S1 or S2 only leads to partially increased resistance. The S6RNAi was also found conferring strong resistance to southern rice black‐streaked dwarf virus (SRBSDV), a novel species closely related to RBSDV that outbroke recently in Southern China. In particular, no adverse effects on agronomical and developmental traits were found in S6RNAi transgenic lines. The marker‐free transgenic lines with S6RNAi, driven by either maize ubiquitin‐1 promoter or rice rbcS green tissue expression promoter, in elite rice background should have great potential in breeding of resistant varieties to both RBSDV and SRBSDV and provide a basis for further safety evaluation and commercial application.
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