Detection of human telomerase reverse transcriptase mRNA in cells obtained by lavage of the pleura is not associated with worse outcome in patients with stage I/II non-small cell lung cancer: results from Cancer and Leukemia Group B 159902.
Detection of human telomerase reverse transcriptase mRNA in cells obtained by lavage of the pleura is not associated with worse outcome in patients with stage I/II non-small cell lung cancer: results from Cancer and Leukemia Group B 159902.
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通过胸膜灌洗获得的细胞中人端粒酶逆转录酶 mRNA 的检测与 I/II 期非小细胞肺癌患者的较差预后无关：来自癌症和白血病组 B 159902 的结果。
DOI：
10.1016/j.jtcvs.2012.08.059
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Kratzke,Robert
中科院分区：
文献类型：
--
作者：
Boylan,AliceM;Wang,XiaofeiF;Ko,Richard;Watson,PatriciaM;Gu,Lin;Harpole,David;Bueno,Raphael;Kelly,Rosemary;Kohman,Leslie;Kratzke,Robert
目的：先前的研究表明，对非小细胞肺癌切除时胸膜表面灌洗获得的细胞进行细胞学分析可以识别有复发风险的患者。由于端粒酶基因表达与非小细胞肺癌的较差预后相关，因此我们假设，鉴定从胸腔灌洗液中获得的表达端粒酶的细胞将识别出有复发疾病风险的患者。方法：疑似非小细胞肺癌患者接受开胸手术以达到治疗目的。使用逆转录酶聚合酶链反应分析通过胸膜表面灌洗获得的细胞的端粒酶催化亚基人端粒酶逆转录酶mRNA表达。结果：共有194例I/II期非小细胞肺癌患者样本充足，中位随访时间为60个月（17-91个月）。通过使用 Cox 模型，人类端粒酶逆转录酶阴性和阳性患者之间的无病生存率（风险比，1.28；95% 置信区间，0.85-1.94；对数秩检验，P = .2349）或总生存率（风险比，1.13；95% 置信区间，0.72-1.79；对数秩检验， P = .5912) 结论：在 I/II 期非小细胞肺癌患者胸腔灌洗液中检测细胞中的人端粒酶逆转录酶并不能识别出有复发风险的患者。
OBJECTIVE: Previous studies suggest that cytologic analysis of cells obtained by lavage of the pleural surfaces at the time of resection of non–small cell lung cancer can identify patients at risk for recurrence. Because telomerase gene expression has been associated with worse outcome in non–small cell lung cancer, we hypothesized that identification of cells obtained from pleural lavage that express telomerase would identify patients at risk for recurrent disease. METHODS: Patients with presumed non–small cell lung cancer underwent thoracotomy with curative intent. Cells obtained by lavage of the pleural surfaces were analyzed for telomerase catalytic subunit human telomerase reverse transcriptase mRNA expression using reverse transcriptase polymerase chain reaction. RESULTS: A total of 194 patients with stage I/II non–small cell lung cancer had adequate samples, and median follow-up was 60 months (17-91 months). By using Cox models, no statistical differences were found between human telomerase reverse transcriptase–negative and positive patients in disease-free survival (hazard ratio, 1.28; 95% confidence interval, 0.85-1.94; log-rank test, P = .2349) or overall survival (hazard ratio, 1.13; 95% confidence interval, 0.72-1.79; log-rank test, P = .5912) CONCLUSIONS: Detection of human telomerase reverse transcriptase in cells obtained from pleural lavage of patients with stage I/II non–small cell lung cancer does not identify patients at risk for recurrent disease.
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DOI：
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发表时间：
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