Detection of telomerase activity in bronchial lavage as an adjunct to cytological diagnosis in lung cancer.
Detection of telomerase activity in bronchial lavage as an adjunct to cytological diagnosis in lung cancer.
复制标题
检测支气管灌洗液中的端粒酶活性作为肺癌细胞学诊断的辅助手段。
DOI：
10.1016/s1010-7940(02)00741-8
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发表时间：
2003
期刊：
影响因子：
--
通讯作者：
P. Doǧan
中科院分区：
文献类型：
--
作者：
E. Dikmen;M. Kara;G. Dikmen;Hüseyin Çakmak;P. Doǧan
目的：肺癌的常规诊断在临床上有时很困难。端粒酶是一种核糖核蛋白DNA聚合酶，在连续的细胞分裂过程中维持染色体的端粒区域。体腔液中端粒酶活性被认为是一种潜在的恶性肿瘤诊断标志物。方法：收集29例经细胞学和/或组织学检查确诊为肺癌的患者支气管灌洗液标本，检测端粒酶活性，分析其对肺癌的诊断价值。患者分为肺癌患者（第1组，n=22）和良性疾病患者（第2组，n=7）。结果：第1组22例中12例（54.5%）细胞学检查确诊，第2组7例全部确诊（P=0.063）。第1组患者中16例（72.7%）端粒酶活性阳性，而第2组中仅1例（14.3%）肺脓肿标本端粒酶活性阳性（P=0.011）。细胞学联合端粒酶活性检测的敏感性（81.8%）明显高于单纯细胞学检测的敏感性（54.5%）（P=0.031）。端粒酶活性检测的敏感性和特异性分别为72.7%和85.7%。端粒酶活性阳性预测值为0.94，阴性预测值为0.50。结论：支气管灌洗液中端粒酶活性检测是一种高度敏感的恶性肿瘤诊断标志物，可作为细胞学检查的补充诊断技术。
Objective: Definitive diagnosis of lung cancer with conventional methods may sometimes be difficult in clinical practice. Telomerase is a ribonucleoprotein DNA polymerase that maintains the telomeric region of chromosomes during successive rounds of cell division. Telomerase activity in body cavity fluids has been advocated to be a potential diagnostic marker for malignancy. We investigated the diagnostic value of telomerase activity in bronchial lavage samples of patients undergoing diagnosis of lung cancer.Methods: A total of 29 bronchial lavage samples were collected from patients in whom the diagnosis was confirmed with cytological and/or histological examinations. Patients were classified as lung cancer patients (Group 1, n=22) and patients with benign disease (Group 2, n=7). Telomerase activity was determined with polymerase chain reaction-based TRAP (The telomeric repeat amplification protocol) assay.Results: Cytological examination was diagnostic in 12 (54.5%) of 22 patients in Group 1, and in all seven patients of Group 2 (P=0.063). Telomerase activity was positive in 16 (72.7%) of Group 1 patients, while it was positive in only 1 (14.3%) sample of a lung abscess in Group 2 (P=0.011). The sensitivity rate of cytological examination when combined with telomerase activity (81.8%) was significantly greater than that of cytological examination alone (54.5%) (P=0.031). The sensitivity and specificity of telomerase activity were 72.7 and 85.7%, respectively. Telomerase activity had a positive predictive value as 0.94 and negative predictive value as 0.50. Diagnostic accuracy of telomerase activity was 75.8%.Conclusion: Telomerase activity in bronchial lavage is a highly sensitive diagnostic biomarker for malignancy and a potential complementary diagnostic technique to cytological examination in the diagnosis of lung cancer.
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影响因子：
4.4
作者：
K. Hiyama;Y. Hirai;S. Kyoizumi;M. Akiyama;E. Hiyama;Mieczyslaw A. Piatyszek;J. Shay;S. Ishioka;M. Yamakido
通讯作者：
K. Hiyama;Y. Hirai;S. Kyoizumi;M. Akiyama;E. Hiyama;Mieczyslaw A. Piatyszek;J. Shay;S. Ishioka;M. Yamakido
影响因子：
56.9
作者：
KIM, NW;PIATYSZEK, MA;SHAY, JW
通讯作者：
SHAY, JW
DOI：
10.1073/pnas.85.18.6622
发表时间：
1988-09-01
影响因子：
11.1
作者：
MOYZIS, RK;BUCKINGHAM, JM;WU, JR
通讯作者：
WU, JR
影响因子：
6.5
作者：
Ramirez, RD;Wright, WE;Taylor, RS
通讯作者：
Taylor, RS
DOI：
10.1093/jnci/87.12.895
发表时间：
1995-06-21
期刊：
JOURNAL OF THE NATIONAL CANCER INSTITUTE
影响因子：
--
作者：
HIYAMA, K;HIYAMA, E;SHAY, JW
通讯作者：
SHAY, JW