Immunohistochemical analysis of the role of hemocytin in nodule formation in the larvae of the silkworm, Bombyx mori.
Immunohistochemical analysis of the role of hemocytin in nodule formation in the larvae of the silkworm, Bombyx mori.
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DOI：
10.1673/031.013.12501
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Sato R
中科院分区：
文献类型：
--
作者：
Arai I;Ohta M;Suzuki A;Tanaka S;Yoshizawa Y;Sato R
血细胞素（Hemocytin）是一种多结构域蛋白。（鳞翅目：天蛾科），是血管性血友病因子的直系同源物，预计是血细胞聚集的主要介质。产生抗血细胞素的抗血清，并对血细胞、血淋巴和结节进行免疫染色。在稳态血细胞中观察到血细胞素，但在血浆中未观察到，即使在细菌注射后。血淋巴涂片可见含血细胞的纤维状结构，形成主要由粒细胞和类酒细胞组成的细胞网络。血细胞素仅在粒细胞的颗粒中染色。当细菌注射后30秒形成结节样聚集体时，观察到粒细胞和细菌细胞都与含血细胞素的纤维结构结合。当结节切片用抗血清染色时，在血细胞周围的结节基质中可见血细胞素。这些数据表明，血细胞素作为从粒细胞胞出的粘性纤维结构的组成部分在结节形成中起着重要作用。
Hemocytin, a multidomain protein from Bombyx mori L. (Lepidoptera: Bombycidae), is an ortholog of von Willebrand factor and is expected to be a major mediator of hemocyte aggregation. Antiserum was generated against hemocytin, and immune staining of hemocytes, hemolymph, and nodules was performed. Hemocytin was observed in steady-state hemocytes but not in plasma, even after bacterial injection. When hemolymph was smeared on glass slides, hemocytin-containing fibrous structures formed a cellular network mainly consisting of granulocytes and oenocytoids. Hemocytin was stained only in the granules of the granulocytes. When nodule-like aggregates formed 30 sec after bacterial injection, both granulocytes and bacterial cells were observed binding to hemocytin-containing fibrous structures. When nodule sections were stained with antiserum, hemocytin was seen in the matrix of the nodules surrounding the hemocytes. These data suggest that hemocytin plays a major role in nodule formation as a component of the sticky fibrous structure exocytosed from granulocytes.
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DOI：
10.1073/pnas.84.14.4846
发表时间：
1987-07-01
影响因子：
11.1
作者：
JENNY, RJ;PITTMAN, DD;MANN, KG
通讯作者：
MANN, KG
DOI：
10.1073/pnas.0306904101
发表时间：
2004-01-27
影响因子：
11.1
作者：
Ariki, S;Koori, K;Kawabata, SI
通讯作者：
Kawabata, SI
影响因子：
4.4
作者：
Gandhe, Archana S.;John, Serene H.;Nagaraju, Javaregowda
通讯作者：
Nagaraju, Javaregowda
影响因子：
2.2
作者：
Koizumi, N;Imai, Y;Sato, R
通讯作者：
Sato, R
DOI：
10.1016/0167-4781(94)00202-e
发表时间：
1995-02-21
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION
影响因子：
--
作者：
KOTANI, E;YAMAKAWA, M;MORI, H
通讯作者：
MORI, H