MRI-based detection of alkaline phosphatase gene reporter activity using a porphyrin solubility switch.
MRI-based detection of alkaline phosphatase gene reporter activity using a porphyrin solubility switch.
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DOI：
10.1016/j.chembiol.2014.01.012
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发表时间：
2014-03-20
影响因子：
--
通讯作者：
Jasanoff A
中科院分区：
文献类型：
--
作者：
Westmeyer GG;Emer Y;Lintelmann J;Jasanoff A
在生物学的许多领域，在活动物中无创地绘制基因表达模式的能力可能会影响。与磁共振成像（MRI）兼容的记者系统可能特别有价值，但是现有的策略往往缺乏灵敏度或特异性。在这里，我们将使用报告酶分泌的碱性磷酸酶（SEAP）与水溶性金属甲磷脂对比剂结合使用基于MRI的基因映射的挑战。 SEAP将卟啉切割成一种不溶性产物，该产物在酶表达的位点积聚，可以通过MRI和光吸光度来观察。对比机制在体外，脑切片和动物中起作用。该系统还提供了在活动物和验尸组织学中读数的可能性，并且显着不需要细胞内递送对比剂。用于检测SEAP的溶解性开关机制可以适应其他报告酶或内源性靶标的成像。
The ability to map patterns of gene expression noninvasively in living animals could have impact in many areas of biology. Reporter systems compatible with magnetic resonance imaging (MRI) could be particularly valuable, but existing strategies tend to lack sensitivity or specificity. Here we address the challenge of MRI-based gene mapping using the reporter enzyme secreted alkaline phosphatase (SEAP), in conjunction with a water soluble metalloporphyrin contrast agent. SEAP cleaves the porphyrin into an insoluble product that accumulates at sites of enzyme expression and can be visualized by MRI and optical absorbance. The contrast mechanism functions in vitro, in brain slices, and in animals. The system also provides the possibility of readout both in the living animal and by post mortem histology, and it notably does not require intracellular delivery of the contrast agent. The solubility switch mechanism used to detect SEAP could be adapted for imaging of additional reporter enzymes or endogenous targets.
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影响因子：
14.9
作者：
DILELLA, AG;HOPE, DA;SMITH, RG
通讯作者：
SMITH, RG
影响因子：
--
作者：
Li Y;Sheth VR;Liu G;Pagel MD
通讯作者：
Pagel MD
影响因子：
3.7
作者：
Badea TC;Hua ZL;Smallwood PM;Williams J;Rotolo T;Ye X;Nathans J
通讯作者：
Nathans J
影响因子：
3.3
作者：
Lelyveld, Victor S.;Atanasijevic, Tatjana;Jasanoff, Alan
通讯作者：
Jasanoff, Alan
影响因子：
15
作者：
Liu, Guanshu;Liang, Yajie;Bar-Shir, Amnon;Chan, Kannie W. Y.;Galpoththawela, Chulani S.;Bernard, Segun M.;Tse, Terence;Yadav, Nirbhay N.;Walczak, Piotr;McMahon, Michael T.;Bulte, Jeff W. M.;van Zijl, Peter C. M.;Gilad, Assaf A.
通讯作者：
Gilad, Assaf A.