Calorie restriction improves metabolic state independently of gut microbiome composition: a randomized dietary intervention trial.
Calorie restriction improves metabolic state independently of gut microbiome composition: a randomized dietary intervention trial.
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DOI：
10.1186/s13073-022-01030-0
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发表时间：
2022-03-14
期刊：
影响因子：
12.3
通讯作者：
Zeller G
中科院分区：
文献类型：
--
作者：
Sowah SA;Milanese A;Schübel R;Wirbel J;Kartal E;Johnson TS;Hirche F;Grafetstätter M;Nonnenmacher T;Kirsten R;López-Nogueroles M;Lahoz A;Schwarz KV;Okun JG;Ulrich CM;Nattenmüller J;von Eckardstein A;Müller D;Stangl GI;Kaaks R;Kühn T;Zeller G
肠道微生物群被认为在超重和肥胖的发展中起着重要作用。然而，热量限制对超重和肥胖成年人肠道微生物群的影响，特别是在较长时间内，在很大程度上尚未探索。在这里，我们纵向分析了间歇性热量限制（ICR）操作为5：2饮食与连续热量限制（CCR）对147名超重或肥胖成年人粪便微生物群的影响，这是一项为期50周的平行臂随机对照试验，HELENA试验。该试验的主要结果是ICR与CCR对皮下脂肪组织基因表达的不同影响。肠道微生物组的变化是本出版物的重点，被定义为试验的探索性终点。该试验包括12周的干预期、12周的维持期和26周的最终随访期。两种饮食均导致约5%的体重减轻。然而，除了在ICR后富集乳杆菌外，干预后微生物组组成在组间没有显著差异。总体体重减轻与代谢的显著改善有关，但与肠道微生物组的变化无关。尽管如此，基线时Dorea属的丰度适度预测随后的体重减轻（AUROC为0.74，用于区分最高与最低体重减轻四分位数）。尽管对微生物组组成缺乏一致的干预作用，但肠道细菌家族与代谢生物标志物、人体测量指标和饮食组成之间的显著研究组独立共变是可检测的。我们的分析特别揭示了胰岛素敏感性（HOMA-IR）与Akkermansiaceae，Christensenellaceae和Tanerellaceae之间的关联。它还表明，通过高蔬菜和高纤维、低加工肉类的饮食，对后两种肠道类群进行有益调节的可能性。总的来说，我们的研究结果表明，在饮食热量限制下，肠道微生物组保持稳定，并且具有高度的个体特异性。该试验（包括当前的微生物组组分）于2015年5月20日在ClinicalTrials.govNCT02449148进行了前瞻性注册。在线版本包含补充材料，可通过10.1186/s13073-022-01030-0获得。
The gut microbiota has been suggested to play a significant role in the development of overweight and obesity. However, the effects of calorie restriction on gut microbiota of overweight and obese adults, especially over longer durations, are largely unexplored. Here, we longitudinally analyzed the effects of intermittent calorie restriction (ICR) operationalized as the 5:2 diet versus continuous calorie restriction (CCR) on fecal microbiota of 147 overweight or obese adults in a 50-week parallel-arm randomized controlled trial, the HELENA Trial. The primary outcome of the trial was the differential effects of ICR versus CCR on gene expression in subcutaneous adipose tissue. Changes in the gut microbiome, which are the focus of this publication, were defined as exploratory endpoint of the trial. The trial comprised a 12-week intervention period, a 12-week maintenance period, and a final follow-up period of 26 weeks. Both diets resulted in ~5% weight loss. However, except for Lactobacillales being enriched after ICR, post-intervention microbiome composition did not significantly differ between groups. Overall weight loss was associated with significant metabolic improvements, but not with changes in the gut microbiome. Nonetheless, the abundance of the Dorea genus at baseline was moderately predictive of subsequent weight loss (AUROC of 0.74 for distinguishing the highest versus lowest weight loss quartiles). Despite the lack of consistent intervention effects on microbiome composition, significant study group-independent co-variation between gut bacterial families and metabolic biomarkers, anthropometric measures, and dietary composition was detectable. Our analysis in particular revealed associations between insulin sensitivity (HOMA-IR) and Akkermansiaceae, Christensenellaceae, and Tanerellaceae. It also suggests the possibility of a beneficial modulation of the latter two intestinal taxa by a diet high in vegetables and fiber, and low in processed meat. Overall, our results suggest that the gut microbiome remains stable and highly individual-specific under dietary calorie restriction. The trial, including the present microbiome component, was prospectively registered at ClinicalTrials.govNCT02449148 on May 20, 2015. The online version contains supplementary material available at 10.1186/s13073-022-01030-0.
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DOI：
10.1016/j.jpba.2017.01.044
发表时间：
2017-05-10
影响因子：
3.4
作者：
Chan, James Chun Yip;Kioh, Dorinda Yan Qin;Chan, Eric Chun Yong
通讯作者：
Chan, Eric Chun Yong
DOI：
10.1038/ismej.2012.8
发表时间：
2012-08
期刊：
The ISME journal
影响因子：
--
作者：
通讯作者：
--
影响因子：
48
作者：
Callahan BJ;McMurdie PJ;Rosen MJ;Han AW;Johnson AJ;Holmes SP
通讯作者：
Holmes SP
影响因子：
82.9
作者：
Depommier, Clara;Everard, Amandine;Cani, Patrice D.
通讯作者：
Cani, Patrice D.
影响因子：
--
作者：
Damms-Machado A;Mitra S;Schollenberger AE;Kramer KM;Meile T;Königsrainer A;Huson DH;Bischoff SC
通讯作者：
Bischoff SC