Independence of chromatin conformation and gene regulation during Drosophila dorsoventral patterning.
Independence of chromatin conformation and gene regulation during Drosophila dorsoventral patterning.
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果蝇背腹纹饰过程中染色质构象的独立性和基因调控。
DOI：
10.1038/s41588-021-00799-x
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发表时间：
2021-04
期刊：
影响因子：
30.8
通讯作者：
Vaquerizas JM
中科院分区：
文献类型：
--
作者：
Ing-Simmons E;Vaid R;Bing XY;Levine M;Mannervik M;Vaquerizas JM
染色质组织与基因调控之间的关系仍不清楚。虽然染色质结构域和结构域边界的破坏可能导致发育基因的错误表达，但基因组组织调节因子的急性耗竭对基因表达的影响相对较小。因此，尚不确定基因表达和染色质状态是否驱动染色质组织，或者染色质组织的变化是否促进细胞类型特异性的基因表达激活。在这里，我们使用果蝇胚胎的背腹模式作为模型系统，为染色质组织和背腹基因表达的独立性提供了证据。我们定义了组织特异性增强子，并使用单细胞 RNA-seq 将它们与表达模式联系起来。令人惊讶的是，尽管存在组织特异性的染色质状态和基因表达，但染色质组织在很大程度上在组织中得以维持。我们的结果表明，组织特异性染色质构象对于组织特异性基因表达不是必需的，而是在增强子变得活跃时充当促进基因表达的支架。果蝇早期胚胎发育过程中，染色质构象很大程度上独立于背腹基因表达。尽管染色质状态和基因表达存在组织特异性差异，但跨组织仍保持三维染色质组织。
The relationship between chromatin organization and gene regulation remains unclear. While disruption of chromatin domains and domain boundaries can lead to misexpression of developmental genes, acute depletion of regulators of genome organization has a relatively small effect on gene expression. It is therefore uncertain whether gene expression and chromatin state drive chromatin organization or whether changes in chromatin organization facilitate cell-type-specific activation of gene expression. Here, using the dorsoventral patterning of the Drosophila melanogaster embryo as a model system, we provide evidence for the independence of chromatin organization and dorsoventral gene expression. We define tissue-specific enhancers and link them to expression patterns using single-cell RNA-seq. Surprisingly, despite tissue-specific chromatin states and gene expression, chromatin organization is largely maintained across tissues. Our results indicate that tissue-specific chromatin conformation is not necessary for tissue-specific gene expression but rather acts as a scaffold facilitating gene expression when enhancers become active. Chromatin conformation is largely independent of dorsoventral gene expression during early embryonic development in Drosophila. Despite tissue-specific differences in chromatin state and gene expression, three-dimensional chromatin organization is maintained across tissues.
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影响因子：
5.4
作者：
Alles J;Karaiskos N;Praktiknjo SD;Grosswendt S;Wahle P;Ruffault PL;Ayoub S;Schreyer L;Boltengagen A;Birchmeier C;Zinzen R;Kocks C;Rajewsky N
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Rajewsky N
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Nollmann M
影响因子：
48
作者：
Amezquita, Robert A.;Lun, Aaron T. L.;Hicks, Stephanie C.
通讯作者：
Hicks, Stephanie C.
影响因子：
64.5
作者：
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