Post-transcriptional control of the Escherichia coli PhoQ-PhoP two-component system by multiple sRNAs involves a novel pairing region of GcvB.
Post-transcriptional control of the Escherichia coli PhoQ-PhoP two-component system by multiple sRNAs involves a novel pairing region of GcvB.
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通过多个SRNA对大肠杆菌phoq-Phop两组分系统的转录后控制涉及GCVB的新型配对区域。
DOI：
10.1371/journal.pgen.1003156
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发表时间：
2013
期刊：
影响因子：
4.5
通讯作者：
Guillier M
中科院分区：
文献类型：
--
作者：
Coornaert A;Chiaruttini C;Springer M;Guillier M
PhoQ/PhoP是一个核心的双组分系统，参与多种细菌的镁稳态、致病性、包膜组成和耐酸性。小RNA GcvB在这里被鉴定为大肠杆菌PhoQ/PhoP合成的一种新的直接调节剂，这种控制依赖于GcvB的一个新的配对区域。继MicA之后，这是第二个抑制phoPQ操纵子表达的hfq依赖性小RNA。MicA和GcvB在体内和体外围绕phoP的翻译起始区与phoPQ mRNA结合。任何一种小RNA的结合都足以抑制核糖体的结合并诱导mRNA降解。然而，令人惊讶的是，云母和GcvB对PhoP蛋白的水平有不同的影响，因此对PhoP调控子的表达有不同的影响。这些结果强调了细菌中小rna和转录调控网络之间的复杂联系。细菌基因表达的调控参与了这些微生物快速适应环境的能力。这种调控可以发生在基因表达的每一个层面。例如，双组分系统参与转录控制，而小rna通常在转录后水平起作用。在本研究中，多效性小RNA GcvB被鉴定为PhoQ/PhoP中枢双组分系统的第二个小RNA调节剂，这突出了不同类型调控之间的联系。
PhoQ/PhoP is a central two-component system involved in magnesium homeostasis, pathogenicity, cell envelope composition, and acid resistance in several bacterial species. The small RNA GcvB is identified here as a novel direct regulator of the synthesis of PhoQ/PhoP in Escherichia coli, and this control relies on a novel pairing region of GcvB. After MicA, this is the second Hfq-dependent small RNA that represses expression of the phoPQ operon. Both MicA and GcvB bind phoPQ mRNA in vivo and in vitro around the translation initiation region of phoP. Binding of either small RNA is sufficient to inhibit ribosome binding and induce mRNA degradation. Surprisingly, however, MicA and GcvB have different effects on the levels of the PhoP protein and therefore on the expression of the PhoP regulon. These results highlight the complex connections between small RNAs and transcriptional regulation networks in bacteria. Regulation of bacterial gene expression participates in the ability of these microorganisms to quickly adapt to their environment. This regulation can occur at every level of gene expression. For instance, two-component systems are involved in transcriptional control, while small RNAs usually act at the post-transcriptional level. In this study, the pleiotropic small RNA GcvB is identified as the second small RNA regulator of the central PhoQ/PhoP two-component system, which highlights the connections between the different types of regulation.
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DOI：
10.1073/pnas.1104318108
发表时间：
2011-09-13
影响因子：
11.1
作者：
Modi, Sheetal R.;Camacho, Diogo M.;Collins, James J.
通讯作者：
Collins, James J.
影响因子：
11.3
作者：
Beisel CL;Storz G
通讯作者：
Storz G
影响因子：
3.6
作者：
Moon K;Gottesman S
通讯作者：
Gottesman S
影响因子：
14.9
作者：
Guillier, Maude;Gottesman, Susan
通讯作者：
Gottesman, Susan
影响因子：
3.2
作者：
Eguchi, Y;Okada, T;Utsumi, R
通讯作者：
Utsumi, R