Conversion of crude oil to methane by a microbial consortium enriched from oil reservoir production waters.
Conversion of crude oil to methane by a microbial consortium enriched from oil reservoir production waters.
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DOI：
10.3389/fmicb.2014.00197
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发表时间：
2014
影响因子：
5.2
通讯作者：
Gieg LM
中科院分区：
文献类型：
--
作者：
Berdugo-Clavijo C;Gieg LM
原油的产甲烷生物降解是发生在石油储层和其它含油环境如污染含水层中的重要过程。在该过程中，互养细菌将烃底物降解成产物，例如乙酸盐和/或H2和CO2，然后产甲烷菌以代谢依赖性方式使用所述产物来产生甲烷。我们从采自低温稠油油藏的生产沃茨中富集了产甲烷原油降解菌群。在培养物中鉴定了指示富马酸添加到C5和C6正烷烃中的烷基琥珀酸盐（高于对照中发现的水平），对应于在培养物中检测到烷基琥珀酸合酶编码基因（assA/masA）。此外，在模拟成熟油田的砂岩填充柱系统中测试富集培养物从残余油产生甲烷的能力。在柱系统中测得的甲烷产率高达5.8 μmol CH 4/g油/天。所产生的甲烷的量与烃损失相对较好地一致，表明饱和烃和芳烃的消耗超过50%。微生物群落分析表明，富集培养物占主导地位的成员属史密斯，Methanosaeta，和Methanoculleus。然而，在砂岩柱中富集培养后，微生物群落发生了变化。在这里，甲烷杆菌属是最丰富的，假单胞菌属的细菌成员和其他已知的生物膜形成生物体也是如此。我们的研究结果表明，从石油储层沃茨中富集的微生物可以生物转化原油组分为甲烷，无论是在厌氧条件下还是在砂岩填充柱中作为测试系统。此外，结果表明，不同的生物可能有助于不同阶段的石油生物降解（例如，无定形与无定形）。
The methanogenic biodegradation of crude oil is an important process occurring in petroleum reservoirs and other oil-containing environments such as contaminated aquifers. In this process, syntrophic bacteria degrade hydrocarbon substrates to products such as acetate, and/or H2 and CO2 that are then used by methanogens to produce methane in a thermodynamically dependent manner. We enriched a methanogenic crude oil-degrading consortium from production waters sampled from a low temperature heavy oil reservoir. Alkylsuccinates indicative of fumarate addition to C5 and C6 n-alkanes were identified in the culture (above levels found in controls), corresponding to the detection of an alkyl succinate synthase encoding gene (assA/masA) in the culture. In addition, the enrichment culture was tested for its ability to produce methane from residual oil in a sandstone-packed column system simulating a mature field. Methane production rates of up to 5.8 μmol CH4/g of oil/day were measured in the column system. Amounts of produced methane were in relatively good agreement with hydrocarbon loss showing depletion of more than 50% of saturate and aromatic hydrocarbons. Microbial community analysis revealed that the enrichment culture was dominated by members of the genus Smithella, Methanosaeta, and Methanoculleus. However, a shift in microbial community occurred following incubation of the enrichment in the sandstone columns. Here, Methanobacterium sp. were most abundant, as were bacterial members of the genus Pseudomonas and other known biofilm forming organisms. Our findings show that microorganisms enriched from petroleum reservoir waters can bioconvert crude oil components to methane both planktonically and in sandstone-packed columns as test systems. Further, the results suggest that different organisms may contribute to oil biodegradation within different phases (e.g., planktonic vs. sessile) within a subsurface crude oil reservoir.
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影响因子：
11.4
作者：
Gieg, LM;Suflita, JM
通讯作者：
Suflita, JM
影响因子：
3
作者：
Grossi, Vincent;Cravo-Laureau, Cristiana;Hirschler-Rea, Agnes
通讯作者：
Hirschler-Rea, Agnes
影响因子：
4.4
作者：
EDWARDS, EA;GRBICGALIC, D
通讯作者：
GRBICGALIC, D
影响因子：
5.1
作者：
Gray ND;Sherry A;Grant RJ;Rowan AK;Hubert CR;Callbeck CM;Aitken CM;Jones DM;Adams JJ;Larter SR;Head IM
通讯作者：
Head IM
影响因子：
3.7
作者：
Gutierrez T;Berry D;Yang T;Mishamandani S;McKay L;Teske A;Aitken MD
通讯作者：
Aitken MD