Glucose-driven TOR-FIE-PRC2 signalling controls plant development.
Glucose-driven TOR-FIE-PRC2 signalling controls plant development.
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DOI：
10.1038/s41586-022-05171-5
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发表时间：
2022-09
期刊：
影响因子：
64.8
通讯作者：
Sheen, Jen
中科院分区：
文献类型：
--
作者：
Ye, Ruiqiang;Wang, Meiyue;Du, Hao;Chhajed, Shweta;Koh, Jin;Liu, Kun-hsiang;Shin, Jinwoo;Wu, Yue;Shi, Lin;Xu, Lin;Chen, Sixue;Zhang, Yijing;Sheen, Jen
营养物质和能量已经成为植物和动物发育程序的中心调节器。进化上保守的雷帕霉素靶激酶（TOR）是控制生长的营养和能量信号的主要整合者。尽管它在翻译、增殖、代谢和自噬中起着重要的调节作用，但人们对TOR如何影响发育转变和分化知之甚少。在这里，我们发现葡萄糖激活的TOR激酶控制全基因组组蛋白H3三甲基化在K27 (H3K27me3)，调节细胞命运和发育。我们发现受精独立胚乳（FIE）是催化H3K27me3的Polycomb抑制复合体2 （PRC2）不可或缺的组成部分，是一个令人惊讶的TOR靶点。直接TOR磷酸化促进FIE动态细胞质到细胞核的易位。靶向FIE磷酸化突变破坏了全球H3K27me3景观，重新编程转录组，并破坏植物的器官发生。此外，葡萄糖- tor - 5 - prc2信号传导调节春化诱导的花转变。我们提出，这个新定义的信号轴作为一个营养检查点，导致关键转录因子基因的表观遗传沉默，这些转录因子基因在茎和根分生组织中指定干细胞命运，并控制叶、花和茎的模式、分支和营养到生殖的转变。这些发现揭示了营养信号在直接表观基因组重编程中的基本机制，在多细胞生物的发育控制中具有广泛的相关性。
Nutrients and energy have emerged as central modulators of developmental programs in plants and animals. The evolutionarily conserved target-of-rapamycin (TOR) kinase is a master integrator of nutrient and energy signalling that controls growth. Despite its important regulatory roles in translation, proliferation, metabolism, and autophagy, little is known about how TOR shapes developmental transitions and differentiation. Here we show that glucose-activated TOR kinase controls genome-wide histone H3 trimethylation at K27 (H3K27me3) that regulates cell fates and development. We identify FERTILIZATION-INDEPENDENT-ENDOSPERM (FIE), an indispensable component of Polycomb repressive complex 2 (PRC2) catalysing H3K27me3, as a surprising TOR target. Direct TOR phosphorylation promotes dynamic cytoplasm-to-nucleus translocation of FIE. The targeted FIE phosphorylation mutation abrogates global H3K27me3 landscape, reprograms transcriptome, and disrupts organogenesis in plants. Moreover, glucose-TOR-FIE-PRC2 signalling modulates vernalization-induced floral transition. We propose that this newly defined signalling axis serves as a nutritional checkpoint leading to epigenetic silencing of key transcription factor genes that specify stem-cell destiny in shoot and root meristems and control leaf, flower and silique patterning, branching, and vegetative-to-reproduction transition. The findings reveal a fundamental mechanism of nutrient signalling in direct epigenome reprogramming with broad relevance in the developmental control of multicellular organisms.
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影响因子：
12.3
作者：
Buels R;Yao E;Diesh CM;Hayes RD;Munoz-Torres M;Helt G;Goodstein DM;Elsik CG;Lewis SE;Stein L;Holmes IH
通讯作者：
Holmes IH
影响因子：
4.5
作者：
Bouyer D;Roudier F;Heese M;Andersen ED;Gey D;Nowack MK;Goodrich J;Renou JP;Grini PE;Colot V;Schnittger A
通讯作者：
Schnittger A
影响因子：
8.8
作者：
Forzani, Celine;Duarte, Gustavo T.;Meyer, Christian
通讯作者：
Meyer, Christian
影响因子：
64.8
作者：
Hsieh, Andrew C.;Liu, Yi;Edlind, Merritt P.;Ingolia, Nicholas T.;Janes, Matthew R.;Sher, Annie;Shi, Evan Y.;Stumpf, Craig R.;Christensen, Carly;Bonham, Michael J.;Wang, Shunyou;Ren, Pingda;Martin, Michael;Jessen, Katti;Feldman, Morris E.;Weissman, Jonathan S.;Shokat, Kevan M.;Rommel, Christian;Ruggero, Davide
通讯作者：
Ruggero, Davide
影响因子：
7.2
作者：
Clough, SJ;Bent, AF
通讯作者：
Bent, AF