Nutrient Supply Is Essential for Shifting Tree Peony Reflowering Ahead in Autumn and Sugar Signaling Is Involved.
Nutrient Supply Is Essential for Shifting Tree Peony Reflowering Ahead in Autumn and Sugar Signaling Is Involved.
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营养供应是牡丹秋季提前复花的必要条件，糖信号参与其中。
DOI：
10.3390/ijms23147703
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发表时间：
2022-07-12
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
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牡丹花期短且集中在春季，限制了牡丹产业的发展。我们以前实现了牡丹在秋季开花。在此，我们通过适当的赤霉素（GA）处理和营养供应，进一步提前其再开花时间。GA单独处理启动芽分化，但它失败后，而GA+营养（G + N）处理提前38天完成开放过程。通过对芽分化和淀粉粒的显微结构观察，我们认为GA在成花诱导中起触发作用，而营养物质的供应则保证了芽的持续发育直至最终开放，两者都是必需的。我们进一步确定了五个成花诱导途径基因的表达，发现PsSOC 1和PsLFY可能分别在成花诱导和营养供应中发挥关键作用。考虑到GA信号，PsGA2ox可能主要参与GA调节，而PsGAI可能进一步调节营养施用后的花形成。此外，G + N处理在早期抑制了糖信号的关键限制酶PsTPS1的表达，表明糖信号也参与了这一过程，而GA单独处理则没有。此外，GA处理诱导了PsSnRK1的高表达，PsSnRK1是一种主要的营养不足指标，PsHXK1是一种合成糖信号物质的限速酶，进一步证实了营养素短缺。总之，在现有的促成栽培技术条件下，除了施用赤霉素外，外源养分的供应是促使牡丹秋季提前开花的关键。
The flowering time of tree peony is short and concentrated in spring, which limits the development of its industry. We previously achieved tree peony reflowering in autumn. Here, we further shifted its reflowering time ahead through proper gibberellin (GA) treatment plus nutrient supply. GA treatment alone initiated bud differentiation, but it aborted later, whereas GA plus nutrient (G + N) treatment completed the opening process 38 days before the control group. Through microstructural observation of bud differentiation and starch grains, we concluded that GA plays a triggering role in flowering induction, whereas the nutriment supply ensured the continuous developing for final opening, and both are necessary. We further determined the expression of five floral induction pathway genes and found that PsSOC1 and PsLFY probably played key integral roles in flowering induction and nutrient supply, respectively. Considering the GA signaling, PsGA2ox may be mainly involved in GA regulation, whereas PsGAI may regulate further flower formation after nutrient application. Furthermore, G + N treatment, but not GA alone, inhibited the expression of PsTPS1, a key restricting enzyme in sugar signaling, at the early stage, indicating that sugar signaling is also involved in this process; in addition, GA treatment induced high expression of PsSnRK1, a major nutrient insufficiency indicator, and the induction of PsHXK1, a rate-limiting enzyme for synthesis of sugar signaling substances, further confirmed the nutrient shortage. In short, besides GA application, exogenous nutrient supply is essential to shift tree peony reflowering ahead in autumn under current forcing culture technologies.
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