Anthocyanidins-enriched bilberry extracts inhibit 3T3-L1 adipocyte differentiation via the insulin pathway.
Anthocyanidins-enriched bilberry extracts inhibit 3T3-L1 adipocyte differentiation via the insulin pathway.
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DOI：
10.1186/1743-7075-8-14
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发表时间：
2011-03-08
影响因子：
4.5
通讯作者：
Kuroda M
中科院分区：
文献类型：
--
作者：
Suzuki R;Tanaka M;Takanashi M;Hussain A;Yuan B;Toyoda H;Kuroda M
肥胖和代谢综合征是重要的公众关注，并且对有效的治疗策略的需求日益增加。预计黄酮类化合物可改善与代谢综合征相关的风险因素。花青素是一种黄酮类化合物，具有抗氧化、抗炎和抗肿瘤的作用。然而，它们对脂肪细胞和分子系统的影响还没有很好的定义。在这项研究中，我们研究了富含花青素的越橘提取物对脂肪细胞分化的影响。利用3 T3-L1细胞系，我们研究了越桔提取物和花青素诱导抑制脂肪形成过程中的脂质积累。为了确定什么是最重要的越橘介导的效果，我们分析了与脂肪细胞分化的关键转录因子的表达真实的时间（RT）-PCR。根据RT-PCR的结果，我们假设越桔提取物和花青素阻断了胰岛素信号，我们通过Western blotting分析确定了胰岛素受体底物1（IRS 1）蛋白酪氨酸残基的磷酸化。此外，我们还通过基因芯片分析和基因集富集分析（GSEA）比较了四种不同生长条件（DMSO、越橘、两种花青素）下脂肪细胞分化早期的全基因组表达谱。在脂肪细胞分化过程中暴露于越橘提取物和花青素抑制3 T3-L1分化。在此期间，越橘提取物和花青素显著降低了一个关键的脂肪细胞分化相关标志物，过氧化物酶体增殖物激活受体- γ（Ppar γ）和固醇调节元件结合蛋白1c（Srebp 1c）。Western blotting分析表明，越橘提取物和花青素降低了IRS 1酪氨酸残基的磷酸化。此外，微阵列实验和GSEA数据显示，在早期分化阶段用越橘提取物或花青素处理的细胞中，胰岛素途径的已知基因的表达显著改变。我们的数据表明，富含花青素的越橘提取物通过胰岛素途径强烈抑制脂肪细胞分化。此外，越橘提取物可能作为一种潜在的补充治疗肥胖患者的代谢综合征。
Obesity and metabolic syndrome are important public concerns, and there is increasing demand for effective therapeutic strategies. Flavonoids are expected to improve the risk factors associated with metabolic syndrome. Anthocyanidins are a kind of flavonoids; well known for their anti-oxidative, anti-inflammatory and anti-tumor properties. However, their effects on adipocytes and molecular systems are not well defined. In this study, we examined the effects of anthocyanidins-enriched bilberry extracts on adipocyte differentiation. Utilizing 3T3-L1 cell line, we investigated that bilberry extracts and anthocyanidins induced inhibition of lipid accumulation during adipogenesis. To identify what is the most important bilberry mediated-effect, we analyzed the expressions of key transcriptional factors associated with adipocyte differentiation by Real Time (RT)-PCR. From the results of RT-PCR, we hypothesized that bilberry extracts and anthocyanidins blocks insulin signal, we determined the phosphorylation of tyrosine residues of insulin receptor substrate 1 (IRS1) protein by western blotting analysis. In addition, we compared the whole-genome expression profiles of early stage of adipocyte differentiation under four different growth conditions (DMSO, bilberry, two anthocyanidins) by microarray analyses and Gene Set Enrichment Analysis (GSEA). Exposure to bilberry extracts and anthocyanidins during adipocyte differentiation inhibited 3T3-L1 differentiation. During this period, bilberry extracts and anthocyanidin significantly decreased a key adipocyte differentiation-associated marker, peroxisome proliferator-activated receptor- γ (Ppar γ ) and sterol regulatory element-binding protein 1c (Srebp1c). Western blotting analysis showed that bilberry extracts and anthocyanidin decreased the phosphorylation of tyrosine residues of IRS1. In addition, microarray experiments and GSEA data revealed significantly altered expression of the known genes of the insulin pathway in cells treated with bilberry extracts or anthocyanidins in the early differentiation stages. Our data demonstrate that anthocyanidin enriched bilberry extracts strongly inhibit the adipocyte differentiation via the insulin pathway. Furthermore, bilberry extracts might be used as a potential complementary treatment for the obese patients with metabolic syndrome.
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影响因子：
10.5
作者：
Kim, JB;Spiegelman, BM
通讯作者：
Spiegelman, BM
影响因子：
4.2
作者：
Takikawa, Masahiro;Inoue, Seiya;Tsuda, Takanori
通讯作者：
Tsuda, Takanori
影响因子：
5.6
作者：
Montagut, Gemma;Blade, Cinta;Ardevol, Anna
通讯作者：
Ardevol, Anna
影响因子：
5.2
作者：
Zafra-Stone, Shirley;Yasmin, Taharat;Bagchi, Debasis
通讯作者：
Bagchi, Debasis
DOI：
10.1042/bj20091112
发表时间：
2009-12-14
期刊：
The Biochemical journal
影响因子：
--
作者：
Payne VA;Au WS;Lowe CE;Rahman SM;Friedman JE;O'Rahilly S;Rochford JJ
通讯作者：
Rochford JJ