The Establishment of a Genetic Transformation System and the Acquisition of Transgenic Plants of Oriental Hybrid Lily (Lilium L.).
The Establishment of a Genetic Transformation System and the Acquisition of Transgenic Plants of Oriental Hybrid Lily (Lilium L.).
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东方杂交百合遗传转化体系的建立及转基因植株的获得
DOI：
10.3390/ijms24010782
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发表时间：
2023-01-02
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
--
作者：
百合（Lilium spp.）花形典雅，色彩艳丽，深受人们喜爱。但百合抗逆性差、自繁能力差，在很大程度上限制了其在园林绿化中的应用。此外，转基因技术是改良植物性状的重要手段，但缺乏稳定高效的遗传转化体系仍是制约百合转基因技术发展的重要因素。因此，本研究通过对不同外植体和不同浓度的植物生长调节剂的筛选，建立了良好的百合再生体系。通过对抗生素临界浓度、菌液浓度和侵染时间的筛选，优化了百合遗传转化体系。最后，将同源的百合抗寒基因LlNAC 2和小鳞茎发生基因LaKNOX 1成功转入‘西伯利亚’和‘索邦’，获得了百合转基因株系。结果表明，以‘西伯利亚’的茎轴为外植体，诱导率高达87%。以‘索邦’花丝为外植体，诱导率高达91.7%。同时，在优化的遗传转化体系中，‘西伯利亚’和‘索邦’的转化率均达到60%以上。本研究为提高东方百合的抗病性和繁殖力以及百合的分子育种提供了理论依据和技术支持。
Lily (Lilium spp.) has elegant flowers and beautiful colors, which makes it popular among people. However, the poor stress resistance and self-propagation ability of lily limit its application in landscaping to a great extent. In addition, transgenic technology is an important means to improve plant characteristics, but the lack of a stable and efficient genetic transformation system is still an important factor restricting the development of lily transgenic technology. Therefore, this study established a good lily regeneration system by screening different explants and plant growth regulators of different concentrations. Then, the genetic transformation system of lily was optimized by screening the critical concentration of antibiotics, the concentration of bacterial solution, and the infection time. Finally, the homologous lily cold resistance gene LlNAC2 and bulblet generation gene LaKNOX1 were successfully transferred to ‘Siberia’ and ‘Sorbonne’ to obtain lily transgenic lines. The results showed that when the stem axis was used as explant in ‘Siberia’, the induction rate was as high as 87%. The induction rate of ‘Sorbonne’ was as high as 91.7% when the filaments were used as explants. At the same time, in the optimized genetic transformation system, the transformation rate of ‘Siberia’ and ‘Sorbonne’ was up to 60%. In conclusion, this study provides the theoretical basis and technical support for improving the resistance and reproductive ability of Oriental lily and the molecular breeding of lily.
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