Structural organization of the cytoskeleton in SV40 human corneal epithelial cells cultured on nano- and microscale grooves.
Structural organization of the cytoskeleton in SV40 human corneal epithelial cells cultured on nano- and microscale grooves.
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DOI：
10.1002/sca.20123
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发表时间：
2008-09
期刊：
影响因子：
--
通讯作者：
Albrecht, Ralph M.
中科院分区：
文献类型：
--
作者：
Karuri, Nancy W.;Nealey, Paul F.;Murphy, Christopher J.;Albrecht, Ralph M.
关键词：
人角膜上皮细胞（HCECs）的基底膜具有三维纳米级结构，包括孔隙、突起和纤维，它们可能影响细胞-底物在上覆细胞中的粘附和扩散。我们之前已经证明，纳米和微尺度的沟槽和山脊模式会影响HCECs对名义壁面剪切应力的形态响应和粘附响应。细胞-底物粘附是由粘附受体介导的，粘附受体与细胞外基质成分结合，并将细胞骨架（CSK）锚定在细胞外成分上。在这里，我们研究了sv40转化的hcec在纳米和微尺度槽和脊模式下的CSK组织。利用x射线光刻技术制备了尺寸从200 nm到2 μm的均匀凹槽和脊状图案。利用扫描电镜和透射电镜对CSK结构和−β1整合素粘附受体的分布进行了研究。与图案对齐的CSK元素；然而，这些元素的空间组织受到特征尺寸的影响。较大的CSK束位于脊的顶部，与图案平行，而较小的CSK束，其宽度与凹槽大小成正比，跨越凹槽。−β1整合素与CSK共定位，在排列的纺锤形细胞的两极具有较高的密度。在不同的地形特征尺寸上看到的组织差异可能表明细胞外基质组织的差异。这在一定程度上可以解释先前关于细胞粘附反应依赖于衬底地形特征大小的观察。
The basement membrane of human corneal epithelial cells (HCECs) has a three-dimensional nanoscale architecture, which includes pores, bumps and fibers that may influence cell–substrate adhesion and spreading in the overlying cells. We previously demonstrated that nano- and microscale groove and ridge patterns influence the morphological response and the adhesive response of HCECs to a nominal wall shear stress. Cell–substrate adhesion is mediated by adhesion receptors that bind to extracellular matrix components and anchor the cytoskeleton (CSK) of cells to extracellular elements. Here we investigate the CSK organization in SV40-transformed HCECs grown on nano- and microscale groove and ridge patterns. X-ray lithography was used to fabricate uniform groove and ridge patterns with features ranging in size from 200 nm to 2 μm grooves. Scanning electron microscopy and transmission electron microscopy were used to investigate CSK structure and the distribution of −β1 integrin adhesion receptors. CSK elements aligned with the patterns; however, the spatial organization of these elements was influenced by feature size. Larger CSK bundles lay on top of the ridges and ran parallel to the patterns, whereas smaller CSK bundles, whose width was proportional to the groove size, spanned the grooves. −β1 integrins co-localized with the CSK and had a higher density at the poles of aligned spindle-shaped cells. Differences in organization seen on the different topographical feature sizes may be indicative of differences in extracellular matrix organization. This may explain, in part, previous observations regarding the dependence of cell adhesive responses on the size of topographic features in the substrate.
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影响因子：
4
作者：
Teixeira, AI;Abrams, GA;Nealey, PF
通讯作者：
Nealey, PF
细胞骨架成分的结构相互作用。
影响因子：
7.8
作者：
Schliwa, M;van Blerkom, J
通讯作者：
van Blerkom, J
影响因子：
4
作者：
Karuri, NW;Liliensiek, S;Murphy, CJ
通讯作者：
Murphy, CJ
影响因子：
4.7
作者：
Huang, Maosheng;Spitz, Margaret R.;Wu, Xifeng
通讯作者：
Wu, Xifeng
影响因子：
--
作者：
WOJCIAKSTOTHARD, B;CURTIS, ASG;WILKINSON, CDW
通讯作者：
WILKINSON, CDW