Single-cell RNA-sequencing profiles reveal the developmental landscape of the Manihot esculenta Crantz leaves.
Single-cell RNA-sequencing profiles reveal the developmental landscape of the Manihot esculenta Crantz leaves.
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DOI：
10.1093/plphys/kiad500
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发表时间：
2023-12-30
期刊：
影响因子：
7.4
通讯作者：
Min, Yi
中科院分区：
文献类型：
--
作者：
Zang, Yuwei;Pei, Yechun;Cong, Xinli;Ran, Fangfang;Liu, Liangwang;Wang, Changyi;Wang, Dayong;Min, Yi
木薯(Manihotesculenta Crantz)是一种重要的光合作用和高产作物。根据其光合作用和结构特征，它被归类为C3-C4植物。为了在细胞水平上研究木薯叶片的结构和光合作用特性，我们建立了木薯叶片的单细胞转录组图谱。共有11,177个优质叶片细胞被划分为15个细胞簇。根据叶细胞标记基因，我们鉴定了木薯叶片的3个主要组织，即叶肉组织、表皮组织和维管组织，并分析了它们的特性和代谢活性。为了补充用于叶细胞类型鉴定的基因，我们筛选了120个候选标记基因。构建了叶细胞发育轨迹图，发现了6个与细胞分化命运相关的基因。从单细胞水平分析木薯叶片的结构和光合作用特性，为进一步提高木薯产量和营养提供理论依据。木薯叶片的单细胞精细图谱揭示了各种类型的细胞特征和功能。
Cassava (Manihot esculenta Crantz) is an important crop with a high photosynthetic rate and high yield. It is classified as a C3–C4 plant based on its photosynthetic and structural characteristics. To investigate the structural and photosynthetic characteristics of cassava leaves at the cellular level, we created a single-cell transcriptome atlas of cassava leaves. A total of 11,177 high-quality leaf cells were divided into 15 cell clusters. Based on leaf cell marker genes, we identified 3 major tissues of cassava leaves, which were mesophyll, epidermis, and vascular tissue, and analyzed their distinctive properties and metabolic activity. To supplement the genes for identifying the types of leaf cells, we screened 120 candidate marker genes. We constructed a leaf cell development trajectory map and discovered 6 genes related to cell differentiation fate. The structural and photosynthetic properties of cassava leaves analyzed at the single cellular level provide a theoretical foundation for further enhancing cassava yield and nutrition. A single-cell fine map of cassava leaves reveals various types of cell characteristics and functions.
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影响因子：
6.9
作者：
Czerednik A;Busscher M;Bielen BA;Wolters-Arts M;de Maagd RA;Angenent GC
通讯作者：
Angenent GC
影响因子：
3.5
作者：
Guerra DD;Pratelli R;Kraft E;Callis J;Pilot G
通讯作者：
Pilot G
影响因子：
3.1
作者：
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通讯作者：
Guo L
影响因子：
0.4
作者：
Bomfim, N.;Ribeiro, D. G.;Nassar, N. M. A.
通讯作者：
Nassar, N. M. A.
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影响因子：
6.5
作者：
Chen, Yang-Er;Ma, Jie;Yuan, Shu
通讯作者：
Yuan, Shu