Reactive oxygen species mediated bacterial biofilm inhibition via zinc oxide nanoparticles and their statistical determination.
Reactive oxygen species mediated bacterial biofilm inhibition via zinc oxide nanoparticles and their statistical determination.
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活性氧物种通过氧化锌纳米颗粒及其统计确定介导细菌生物膜抑制。
DOI：
10.1371/journal.pone.0111289
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Al-Khedhairy AA
中科院分区：
文献类型：
--
作者：
Dwivedi S;Wahab R;Khan F;Mishra YK;Musarrat J;Al-Khedhairy AA
细菌生物被膜的形成是临床应用中的主要挑战。本研究的主要目的是描述氧化锌纳米颗粒（NPs）的合成，表征和对细菌菌株铜绿假单胞菌的生物杀灭潜力。这些纳米粒子的合成通过软化学溶液过程中在很短的时间内，其结构特性已被详细研究，通过使用X射线衍射和透射电子显微镜测量。在这项工作中，合成的ZnO-NP（μ m 10-15 nm）在体外抑制细菌的潜力得到了评估，并使用组织培养板试验观察了其生物膜的形成。结晶紫染色生物膜形成及其光密度显示生物膜抑制的效果。浓度为100 μg/mL的NP显著抑制细菌生长和生物膜形成。通过生物透射电子显微镜（Bio-TEM）也证实了ZnO-NPs的生物膜抑制。ZnO纳米粒子处理的细菌的生物透射电镜分析证实了细胞的变形和损伤。在NP存在下的细菌生长以浓度依赖性方式推断NP的杀菌能力。据推测，纳米颗粒作为表面涂层材料的抗菌活性可能是控制病原体的可行方法。此外，所获得的菌液数据也与统计分析方法的结果一致。
The formation of bacterial biofilm is a major challenge in clinical applications. The main aim of this study is to describe the synthesis, characterization and biocidal potential of zinc oxide nanoparticles (NPs) against bacterial strain Pseudomonas aeruginosa. These nanoparticles were synthesized via soft chemical solution process in a very short time and their structural properties have been investigated in detail by using X-ray diffraction and transmission electron microscopy measurements. In this work, the potential of synthesized ZnO-NPs (∼10–15 nm) has been assessed in-vitro inhibition of bacteria and the formation of their biofilms was observed using the tissue culture plate assays. The crystal violet staining on biofilm formation and its optical density revealed the effect on biofilm inhibition. The NPs at a concentration of 100 µg/mL significantly inhibited the growth of bacteria and biofilm formation. The biofilm inhibition by ZnO-NPs was also confirmed via bio-transmission electron microscopy (Bio-TEM). The Bio-TEM analysis of ZnO-NPs treated bacteria confirmed the deformation and damage of cells. The bacterial growth in presence of NPs concluded the bactericidal ability of NPs in a concentration dependent manner. It has been speculated that the antibacterial activity of NPs as a surface coating material, could be a feasible approach for controlling the pathogens. Additionally, the obtained bacterial solution data is also in agreement with the results from statistical analytical methods.
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影响因子：
7.6
作者：
Mishra, Yogendra Kumar;Adelung, Rainer;Roehl, Claudia;Shukla, Deepak;Spors, Frank;Tiwari, Vaibhav
通讯作者：
Tiwari, Vaibhav
影响因子：
7.4
作者：
Contado, Catia;Pagnoni, Antonella
通讯作者：
Pagnoni, Antonella
零紫bismuth纳米颗粒抑制链球菌的生长和生物膜的形成。
影响因子：
8
作者：
Hernandez-Delgadillo R;Velasco-Arias D;Diaz D;Arevalo-Niño K;Garza-Enriquez M;De la Garza-Ramos MA;Cabral-Romero C
通讯作者：
Cabral-Romero C
影响因子：
3.9
作者：
Eshed, Michal;Lellouche, Jonathan;Banin, Ehud
通讯作者：
Banin, Ehud
影响因子：
3.8
作者：
FRESTA, M;PUGLISI, G;FURNERI, PM
通讯作者：
FURNERI, PM